蜂斗菜不同提取物对豚鼠离体回肠收缩的影响

Effect of Different Extracts of Petasites Japonicus on the Contraction of Isolated Ileum of Guinea Pig

关键词:  蜂斗菜  豚鼠离体回肠  组胺  乙酰胆碱

Key words:  Petasites japonicus  isolated guinea pig ileum  histamine  acetylcholine

摘要:

目的 比较蜂斗菜不同提取物对豚鼠离体回肠收缩的影响。方法 采用豚鼠离体回肠实验，以组胺、乙酰胆碱不同激动剂为研究模型，比较蜂斗菜CO₂超临界提取物、80%乙醇提取物、80%乙醇提取物的不同极性提取物及乙酸乙酯提取物经硅胶柱层析分离获得的不同馏分对豚鼠离体回肠的收缩作用，计算各个提取物对豚鼠回肠收缩幅度的抑制率。结果 蜂斗菜不同提取物对组胺、乙酰胆碱所致的豚鼠离体回肠收缩有显著拮抗作用，其中乙酸乙酯提取物硅胶柱色谱的馏分抑制作用最强。结论 蜂斗菜不同提取物均能抑制组胺、乙酰胆碱引起的豚鼠离体回肠收缩作用，脂溶性成分抑制作用最强，其机制可能与M、H₁受体的抑制有关。

Abstract:

OBJECTIVE To compare the effect of different extracts of Petasites japonicus on the contraction of isolated ileum of guinea pig. METHODS By using isolated guinea pig ileum experiments, histamine, acetylcholine load model were used as research models. Petasites japonicus was extracted by supercritical CO₂ and 80% ethanol respectively. The 80% ethanol extract was fracted by petroleum, ethyl acetate, n-butyl alcohol. The ethyl acetate extract was purified by silica gel column chromatography. Inhibition ratio of the contraction extent was calculated. RESULTS Different extracts of Petasites japonicus remarkably inhibited the contraction caused by histamine and acetylcholine. Ethyl acetate extract which was purified by silica gel column chromatography had the strongest inhibitory effect. CONCLUSION The different extracts of Petasites japonicus significantly inhibited the contraction of isolated ileum of guinea pig caused by histamine and acetylcholine. The effect of Petasites japonicus on the contraction of isolated ileum of guinea pig was fat-soluble components and was highly relevant to M ACh-receptors and H₁ receptors.