星点设计-效应面法优化龙葵生物碱提取工艺

Optimization of Extraction Process of Alkaloid from Solanum Nigrum by Central Composite Design-response Surface Methodology

关键词:  龙葵  生物碱  星点设计  效应面法

Key words:  Solanum nigrum  alkaloid  central composite design  response surface methodology

摘要:

目的 通过星点设计-效应面法优化龙葵生物碱提取工艺。方法 在单因素试验的基础上以提取液中95%乙醇体积分数、提取温度和提取时间为考察因素，以澳洲茄碱提取率为考察指标，采用星点设计-效应面法设计试验优化龙葵生物碱的提取工艺。结果 经运用DPS软件处理，选用3次多项式模型描述考察指标和3个考察因素之间的数学关系，并绘制效应面，确定最佳提取工艺参数：95%乙醇体积分数为73.6%，提取温度为60 ℃，提取时间为63.3 min，澳洲茄碱提取率预测值为0.683 0 mg·g^-1，验证值为0.676 3 mg·g^-1。结论 星点设计-效应面法能有效优化龙葵生物碱提取工艺，预测性良好。

Abstract:

OBJECTIVE To optimize the extraction process for alkaloid by the central composite design-response surface methodology from Solanum nigrum. METHODS On the basis of one factor tests, the method of the central composite design-response surface analysis 3 factors including concentration of methanol in the extraction impregnate, extraction temperature, time, and extraction percentage of alkaloid from Solanum nigrum as response value was adopted. RESULTS The quantitative relationships between 3 factors and the response value were characterized by DPS. Optimal formulation was proposed to concentration of methanol 73.6%, extraction temperature 60 ℃, extraction time 63.3 min. The predicted value and measured value of alkaloid from Solanum nigrum was 0.683 0 mg·g^-1 and 0.676 3 mg·g^-1, respectively. CONCLUSION The result indicates that extraction process of alkaloid from Solanum nigrum could be achieved by the central composite design-response surface methodology, and it is predictable.