| 引用本文: | 李嘉,张颖,姜平川,张赟赟.广西不同产地壮药玉叶金花中Mussaendoside G的含量测定[J].中国现代应用药学,2014,31(12):1478-1480. |
| LI JIA,ZHANG Ying,JIANG Pingchuan,ZHANG Yunyun.Determination of Mussaendoside G in Mussaenda Pubescens in Different Areas of Guangxi Province by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(12):1478-1480. |
|
| 摘要: |
| 目的 建立高效液相色谱法测定玉叶金花中mussaendoside G的含量。方法 色谱柱为Techmate C18(4.6 mm× 250 mm，5 μm)，流动相：乙腈-水(38∶62)，检测波长：265 nm，流速：1.0 mL·min-1。结果 mussaendoside G在0.06~ 0.60 mg·mL-1(r=0.999 9)内线性关系良好，平均回收率为99.34%，RSD为2.32%。不同产地玉叶金花中mussaendoside G的含量相差较大。结论 此方法简便、快捷、准确，可用于玉叶金花药材的质量控制。 |
| 关键词: mussaendoside G 玉叶金花 含量测定 高效液相色谱法 |
| DOI： |
| 分类号: |
| 基金项目:广西壮族自治区科技厅项目(桂科能12239004-2) |
|
| Determination of Mussaendoside G in Mussaenda Pubescens in Different Areas of Guangxi Province by HPLC |
|
LI JIA1,2, ZHANG Ying1,2, JIANG Pingchuan1,2, ZHANG Yunyun1,2
|
|
1.Guangxi Key Laboratory of the Quality Standard of Traditional Chinese Medicine, Nanning 530022, China;2.Department of Chemistry, Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China
|
| Abstract: |
| OBJECTIVE To establish an HPLC method for the simultaneous determination of mussaendoside G in Mussaenda pubescens in different areas of Guangxi province. METHODS Mussaendoside G was separated on Techmate C18 (4.6 mm×250 mm, 5 μm) column and detected at 265 nm. The mobile phase was acetonitrile-water(38∶62). The flow rate was 1.0 mL·min-1. RESULTS Mussaendoside G was 1inear within the range of 0.06-0.60 mg·mL-1(r=0.999 9). The average recovery was 99.34%(RSD=2.32%). The contents of mussaendoside G in different areas were different. CONCLUSION The method is simple, rapid, and it can be used for the quality control of Mussaenda pubescens． |
| Key words: mussaendoside G Mussaenda pubescens content determination HPLC |
