左归降糖解郁方对糖尿病并发抑郁症大鼠行为学及认知功能的影响

Effect of Zuogui Jiangtang Jieyu Fang on Behaviors and Cognitive Function in Diabetes Mellitus Rats with Depression

关键词:  糖尿病并发抑郁症  左归降糖解郁方  促肾上腺皮质激素释放激素  促肾上腺皮质激素

Key words:  diabetes mellitus with depression  Zuogui Jiangtang Jieyu Fang  corticotropin releasing hormone  adrenocorticotropic hormone

摘要:

目的 研究左归降糖解郁方对糖尿病并发抑郁症大鼠行为学及认知功能的影响。方法 建立糖尿病并发抑郁症动物模型并随机分为6组，模型组、二甲双胍(0.18 g·kg^-1)+盐酸氟西汀(1.8 mg·kg^-1)组(阳性药组)、左归降糖解郁方高、中、低剂量(20.53，10.26，5.13 g·kg^-1)组，并设正常对照组。给药42 d后，进行开野和Morris水迷宫行为学评价，采用ELISA法测定各组大鼠血浆促肾上腺激素释放激素(corticotropin releasing hormone，CRH)、促肾上腺皮质激素(adrenocorticotropic hormone，ACTH)含量。结果 与正常对照组比较，模型组大鼠开野实验中活动总数明显减少(P<0.01)，Morris水迷宫实验中逃避潜伏期明显延长(P<0.05或P<0.01)，空间探索时间明显缩短(P<0.05)；血浆CRH、ACTH含量显著升高(P<0.05或P<0.01)。与模型组比较，给予左归降糖解郁方高剂量后，模型大鼠活动次数增多(P<0.05)，学习记忆能力得到一定程度恢复，血浆CRH、ACTH水平明显降低(P<0.05)。结论 左归降糖解郁方可改善糖尿病并发抑郁症模型大鼠的抑郁行为及认知障碍，可能与其降低CRH和ACTH水平，缓解HPA轴亢进有关。

Abstract:

OBJECTIVE To investigate the effect of Zuogui Jiangtang Jieyu Fang on the behaviors and cognitive function in diabetes mellitus rats with depression. METHODS Diabetes mellitus with depression rats were randomly divided into six groups, model group, metformin(0.18 g·kg^-1) combined fluoxetine(1.8 mg·kg^-1) group, Zuogui Jiangtang Jieyu Fang high, medium and low dose(20.53, 10.26, 5.13 g·kg^-1) groups, and control group. After 42 d of administration, open-field test and Morris water maze were used to evaluate the activity of rats. Plasma of CRH and ACTH levels were detected by ELISA method. RESULTS Compared with the control group, the total activities of open-field test activities of rats of model group were significantly reduced(P<0.01), the escape latencies were significantly longer in the Morris water maze test(P<0.05 or P<0.01), as well as the space exploration times were significantly shortened(P<0.05). Plasma levels of CRH and ACTH levels were significantly higher(P<0.05 or P<0.01). Compared with the model group, the locomotor activity of Zuogui Jiangtang Jieyu Fang high group rats were significantly increased(P<0.05), and the learning and memory capacity to be a certain degree of recovery. The plasma levels of CRH and ACTH were significantly decreased(P<0.05). CONCLUSION The behaviors and cognitive function could be improved by Zuogui Jiangtang Jieyu Fang. Its function might be correlated with reducing the levels of CRH and ACTH, and relieving the hyperactivity of HPA axis.