辅助期结肠癌患者脾虚证与湿热证血清蛋白质组学比较研究

Explore Different Expression of Serum Proteome of Colon Cancer Adjuvant Therapy Period in Agglomeration of Dampness and Heat Syndrome and Spleen Deficiency Syndrome

关键词:  结肠癌  湿热证  脾虚证  血清蛋白  蛋白质组学

Key words:  colon cancer  agglomeration of dampness and heat syndrome  spleen deficiency syndrome  serum protein  serum proteomics

摘要:

目的 研究辅助治疗期结肠癌湿热证、脾虚证及无症状结肠癌患者血清差异表达的蛋白质。方法 收集结肠癌辅助期结肠癌湿热证组、脾虚证组及无症状组患者外周血清各8例，通过双向凝胶电泳以及质谱鉴定和生物信息学分析，寻找在结肠癌辅助治疗期患者血清中特异表达的蛋白质。结果 湿热证组较无症状组维生素D结合蛋白升高，血清结合素前体、β-GDP解离抑制因子、载脂蛋白A-I前体和簇蛋白均降低。脾虚证组较无症状组β-GDP解离抑制因子、簇蛋白及血清结合素前体升高，前白蛋白原、载脂蛋白A-I前体及维生素D结合蛋白均降低。结论 6种蛋白差异表达蛋白质的组合性变化可能是辅助治疗期结肠癌的物质基础，具有潜在的诊断、预后标志物或治疗靶点的意义。

Abstract:

OBJECTIVE Analysis serum proteome of constitution of nonsymptomatic, agglomeration of dampness and heat syndrome, and spleen deficiency syndrome in colon cancer. METHODS Two-dimensional difference gel electrophoresis and mass spectrometry were used to screen the differentially expressed plasma proteins among patients with nonsymptomatic group, agglomeration of dampness and heat group, and spleen deficiency group. RESULTS Compared with nonsymptomatic group, vitamin D-binding protein was significantly highly expressed in agglomeration of dampness and heat group, and hemopexin precursor, GDP dissociation inhibitor beta, apolipoprotein A-I precursor, clusterin, vitamin D-binding protein were decreased in their plasma; while GDP dissociation inhibitor beta, clusterin, hemopexin precursor was significantly highly expressed in spleen deficiency group, and apolipoprotein A-I precursor, vitamin D-binding protein, apolipoprotein A-I precursor, and albumin preproprotein were decreased in their plasma. CONCLUSION The different syndrome of palliative therapy period in colon cancer could be attributed to the amount of proteins’ expression. Research technique of “Different syndromes with same disease” is the way to filt the specific object target of syndrome nature.