荧光光谱法研究顺铂对表柔比星与人血白蛋白结合作用的影响

Fluorescent Spectrometry Study of Influence of Cisplatin in Conjugation Between Epirubicin and Human Serum Albumin

关键词:  顺铂  表柔比星  人血白蛋白  荧光猝灭

Key words:  cisplatin  epirubicin  human serum albumin  fluorescence quenching

摘要:

目的 研究顺铂对表柔比星与人血清白蛋白(HSA)结合作用的影响。方法 通过荧光光谱法研究顺铂和表柔比星对HSA的荧光猝灭光谱，同步荧光光谱。由Lineweaver-Burk双倒数作图法确定反应的解离常数，根据热力学方程讨论两者间主要的作用力类型。结果 荧光猝灭光谱显示，顺铂和表柔比星与HSA都有荧光猝灭作用。顺铂、表柔比星对HSA的猝灭过程为静态猝灭。表柔比星与HSA的结合点数为1，主要作用力为疏水作用力。顺铂不影响表柔比星对HSA的内源荧光猝灭作用，但能增加表柔比星与HSA的结合常数(K_A)。结论 顺铂不影响表柔比星的血药浓度，但能增加表柔比星与HSA的结合力。

Abstract:

OBJECTIVE To investigate the interaction between cisplatin and epirubicin in conjugation of human serum albumin(HSA). METHODS The interaction of cisplatin and epirubicin with HSA was studied by measuring fluorescence quenching spectra, synchronous fluorescence spectra. The binding constants were calculated according to Lineweaver-Burk equation and type of binding force was estimated by the calculation of thermodynamic parameters. RESULTS The fluorescence quenching spectra of HSA in the presence of aisplatin and epirubicin showed that both of them quenched the fluorescence of HSA. Cisplatin and epirubicin were static quenching. The binding site numbers of epirubicin was 1. The interaction between epirubicin and HSA was mainly driven by a hydrophobic force. When in the presence of cisplatin, the combining constants (K_A) increased. CONCLUSION Cisplatin can increase the combining constants.