| 引用本文: | 叶丽香,苏燕评,曹大炫,林菁,俞昌喜.UPLC测定钩吻素子的含量及其水溶液稳定性[J].中国现代应用药学,2015,32(4):471-474. |
| YE Lixiang,SU Yanpin,CAO Daxuan,LIN Jing,YU Changxi.Content Determination and Stability of Koumine by UPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(4):471-474. |
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| 摘要: |
| 目的 建立超高效液相色谱法测定钩吻素子的含量，并考察其水溶液在室温条件下的稳定性。方法 采用Agilent Extend-C18(2.1 mm×100 mm，1.8 μm)色谱柱，以乙腈-0.2%正丁胺水溶液(30∶70)为流动相，流速0.5 mL?min-1，检测波长256 nm，测定3个批次钩吻素子的含量，并考察钩吻素子水溶液在室温条件下稳定性。结果 钩吻素子分离度良好；在10.19~112.2 μg?mL-1内线性关系良好(r=0.999 7)，定量限为0.1 μg?mL-1；平均回收率为100.4%(RSD=0.45%，n
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| 关键词: 钩吻素子 超高效液相色谱法 含量测定 稳定性 |
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| 基金项目:福建省自然科学基金资助项目(2013J01374)；福建医科大学重大科研项目计划(09ZD009) |
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| Content Determination and Stability of Koumine by UPLC |
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YE Lixiang1, SU Yanpin2, CAO Daxuan3, LIN Jing4, YU Changxi4
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1.Fujian Center for Safety Evaluation of New Drug, Fujian Medical University Fuzhou, 350108, China;2.College of Pharmacy, Fujian Medical University, Fuzhou 350108, China;3.Fujian Medical University Fujian Center for Safety Evaluation of New Drug, Fuzhou 350108, China;4.Fujian Center for Safety Evaluation of New Drug, College of Pharmacy, Fujian Medical University, Fuzhou 350108, China
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| Abstract: |
| OBJECTIVE To establish a ultra high performance liquid chromatography(UPLC) method for determination of koumine and to study the stability of koumine solution at room temperature. METHODS The analysis was achieved by Agilent Extend-C18 chromatogram column(2.1 mm×100 mm, 1.8 μm). The mobile phase composed of acetonitrile-0.2% butylamine (30∶70) at flow rate of 0.5 mL?min-1. The detection wavelength was set at 256 nm. Three batches of koumine were determined and stability of its solution was examined under room temperature. RESULTS Under the estabilished method, the separation of koumine and its main related substances was good. The linear range of koumine was 10.19-112.2 μg?mL-1(r=0.999 7) with detection limit of 0.1 μg?mL-1. The average recovery was 100.4% (RSD=0.45%, n=9). The average concentration of koumine were 99.97%(n
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| Key words: koumine UPLC content deterrnination stability |
