不同生长发育期味连次生代谢产物积累变化研究

Study on the Dynamic Accumulation of Secondary Metabolites in Coptis Chinensis Franch. in Different Growth and Development Period

关键词:  味连  次生代谢产物  生物碱  生长发育期

Key words:  Coptis chinensis Franch.  secondary metabolites  alkaloids  growth and development period

摘要:

目的 研究重庆石柱道地中药材味连在不同生长发育期的次生代谢产物积累动态变化规律，为确定最佳采收期，实现该地区味连的GAP种植和质量控制提供理论依据。方法 采集不同生长发育期的4年生味连样品，采用HPLC测定表小檗碱、黄连碱、巴马汀和小檗碱的含量。结果 表小檗碱含量在抽葶开花期的最高(1.82%)，冬季休眠期最低(1.33%)；黄连碱含量在新叶盛发期最高(3.86%)，冬季休眠期最低(2.79%)；巴马汀含量在新叶盛发期最高(2.32%)，冬季休眠期最低(1.69%)；小檗碱含量在新叶缓发期最高(10.89%)，冬季休眠期最低(8.28%)。结论 味连次生代谢产物的含量随植株的生长发育而波动，其中新叶盛发期、新叶缓发期和抽葶开花期的生物碱含量高于根茎充实期和冬季休眠期。最佳采收期应结合产量和次生代谢产物含量综合判断。

Abstract:

OBJECTIVE To explore the dynamic accumulation of secondary metabolites in the genuine medicinal plant of Chongqing Coptis chinensis Franch. in different growth period in Shizhu county, and provide an experimental base for determination of optimal harvest time, GAP planting and quality control. METHODS The contents of epiberberine, coptisine, palmatine and berberine of 4-year-old Coptis chinensis Franch. collected in different growth development period were analyzed by HPLC. RESULTS The contet of epiberberine in sprouting stage was the highest(1.82%) and the lowest(1.33%) in winter dormant period; the contet of coptisine was the highest(3.86%) in new leaves flourishing period and the lowest(2.79%) in winter dormant period; the contet of palmatine was the highest(2.32%) in new leaves flourishing period and the lowest(1.69%) in winter dormant period; the contet of berberine was the highest(10.89%) in leaves slowly growing period and the lowest(8.28%) in winter dormant period. CONCLUSION The content of secondary metabolites in Coptis chinensis Franch. fluctuates with its growth and development; and the content of alkaloid in new leaves flourishing period, slowly growing period and sprouting stage is higher than that in the period of rhizome filling and winter dormancy. The optimal harvest time should be determined by both the production and the quality.