怀菊花多成分含量测定及指纹图谱的模式识别研究

Determination of Multi Components and Pattern Recognition of Fingerprint of Huai Chrysanthemi Flos

关键词:  怀菊花  多成分含量测定  指纹图谱  模式识别

Key words:  Huai Chrysanthemi Flos  determination of multi components  fingerprint  pattern recognition

摘要:

目的 建立怀菊花中多种活性成分含量的HPLC检测方法，构建怀菊花的指纹图谱识别模式。方法 采用HPLC检测10批怀菊花样品绿原酸、3,4-二咖啡酰基奎宁酸、3,5-二咖啡酰基奎宁酸、4,5-二咖啡酰基奎宁酸和木犀草素等化合物的含量，建立指纹图谱。运用主成分分析及聚类分析对指纹图谱进行模式识别研究，并采用相似度评价软件进行相似度评价。色谱柱为SWELL Chromplus C₁₈ (250 mm×4.6 mm，5 μm)；流动相为乙腈-0.3%甲酸水溶液，梯度洗脱，流速为0.8 mL·min^-1；进样量：10 μL；检测波长：350 nm；柱温：25 ℃。结果 绿原酸的含量为0.04%~0.27%，3,4-二咖啡酰奎尼酸的含量为0.01%~0.21%，3,5-二咖啡酰奎尼酸的含量为0.45%~1.09%，4,5-二咖啡酰奎尼酸的含量为0.32%~0.96%，木犀草素的含量为0.02%~0.18%。怀菊花的指纹图谱确定了15个共有峰，根据前3个主成分将10批样品分为5类。结论 本方法简便、准确、重现性好，为怀菊花多成分的含量测定提供参考方法，并为怀菊花的质量控制提供科学依据。

Abstract:

OBJECTIVE To establish an analytic method of multi components of Huai Chrysanthemi Flos by HPLC and its related fingerprint pattern recognition. METHODS HPLC was used to measure the content of chlorogenic acid, 3,4-dicaffeoyl puininic acid, 3,5-dicaffeoyl puininic acid, 4,5-dicaffeoyl puininic acid, luteolin and establish the fingerprint of Huai Chrysanthemi Flos. The fingerprint recognition pattern was established by using principle component analysis and clustering analysis based on the results from 10 batches of Huai Chrysanthemums Flos. Subsequent similarity evaluation was conducted by using similarity evaluation software. The column was SWELL Chromplus C₁₈ (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile-0.3% formic acid. Gradient elution was used and its flow rate was 0.8 mL·min^-1. Injection volume was 10 μL. Detection wavelength was 350 nm. Column temperature was 25?℃. RESULTS The average content ranges for chlorogenic acid, 3,4-dicaffeoyl puininic acid, 3,5-dicaffeoyl puininic acid, 4,5-dicaffeoyl puininic acid and luteolin were 0.04%-0.27%, 0.01%-0.21%, 0.45%-1.09%, 0.32%-0.96% and 0.02%-0.18%, respectively. The fingerprint pattern recognition of Huai Chrysanthemi Flos was established, and there were 15 common peaks in the identified fingerprints. The samples were divided into five categories based on the first three principal components extracted in fingerprints. CONCLUSION This method is simple, accurate and reproducible, which can provide a reference for the multi-component determination of Huai Chrysanthemi Flos, thus providing the scientific evidence for its quality control.