芳香新塔花总黄酮对大鼠离体心脏缺血再灌注损伤的保护作用

Effect of Ziziphora Clinopodioides Flavonoids on Myocardial Ischemia Reperfusion Injury in Isolated Rat Hearts

关键词:  芳香新塔花总黄酮  心肌缺血再灌注  血流动力学

Key words:  Ziziphora clinopodioides flavonoids  myocardial ischemia reperfusion  hemodynamics

摘要:

目的 研究芳香新塔花总黄酮(Ziziphora clinopodioides flavonoids，ZCF)对大鼠离体心脏缺血再灌注(I/R)损伤的保护作用。方法 SD大鼠40只，随机分为5组：空白组，I/R组，ZCF低剂量组(12.5 mg?L^-1)，ZCF中剂量组(25 mg?L^-1)，ZCF高剂量组(50 mg?L^-1)，采用Langendorff法建立离体大鼠心脏I/R模型。观察I/R期间室内压最大上升速率(+dp/dt_max)、室内压最大下降速率(-dp/dt_max)、左心室舒张压(LVEDP)、冠脉流量(CF)和心率(HR)。测定心肌组织中肌酸激酶(CK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量。结果 ZCF高剂量组和中剂量组均可显著改善I/R所致的心功能损伤，减少CK、LDH的释放和心肌组织MDA 的产生，增加SOD的活性(与模型组相比，P<0.001，P<0.01或P<0.05)。结论 ZCF对大鼠离体心脏I/R损伤具有保护作用，其作用可能与清除氧自由基、减少脂质过氧化反应、提高心肌抗氧化能力、维持细胞膜的完整性有关。

Abstract:

OBJECTIVE To study the effect of Ziziphora clinopodioides flavonoids(ZCF) on myocardial ischemia reperfusion(I/R) injury in isolated rat hearts. METHODS Fourteen Sprague-Dawley rats were randomly divided into five groups: normal control group, I/R group and ZCF group(12.5 mg?L^-1，25 mg?L^-1，50 mg?L^-1). Langendorff-perfusion isolated rat hearts were prepared. During myocardial ischemia reperfusion, the left cardiac function was measured by the analysis system, such as +dp/dt_max, -dp/dt_max, LVEDP, CF and HR. The activities of creatine kinase(CK), lactate dehydrogenase(LDH) in myocardial tissues were measured to evaluate the injury of myocardial cells and the protective effect of ZCF. Meanwhile, the activity of superoxide dismutase(SOD) and the contents of malondialdehyde(MDA) in myocardial tissues were determined. RESULTS Compared with the I/R group, ZCF 25 and 50 mg?L^-1 could recover cardiac function, and reduce the release of CK, LDH from I/R rat hearts. It could increase the activity of SOD, and decrease the MDA product(P<0.001, P<0.01 or P<0.05). CONCLUSION ZCF possesses protective effect on isolated heart I/R injury. The mechanism may include the remaining of myocardial enzymes activity(CK, LDH ) in cardiac muscle except scavenging oxygen free radical, reducing lipid peroxidation.