反式白藜芦醇对Aβ25-35诱导的阿尔兹海默病小鼠模型学习记忆的改善作用

Effect of Trans-resveratrol on Learning and Memory Abilities in Alzheimer’s Disease Mice Induced by Aβ25-35

关键词:  反式白藜芦醇  β淀粉样肽  阿尔茨海默病  学习记忆  神经可塑性

Key words:  trans-resveratrol  β-amyloid  Alzheimer’s disease  learning and memory  neural plasticity

摘要:

目的 观察反式白藜芦醇对阿尔茨海默病(Alzheimer's disease，AD)模型小鼠记忆损伤的改善作用。方法 小鼠随机分为假手术组、模型组和反式白藜芦醇10，20，40 mg·kg^-1组。在小鼠海马CA1区注射Aβ25-35后给予反式白藜芦醇10 d，采用水迷宫试验观察药物对小鼠学习记忆的影响，免疫组化法观察各组小鼠海马CA1区神经元可塑性变化，western-blot法检测神经可塑性相关蛋白的表达变化。结果 40 mg·kg^-1反式白藜芦醇能明显缩短AD小鼠寻找平台的潜伏期，增加原平台所在象限的停留时间和穿越次数；20，40 mg·kg^-1反式白藜芦醇能增加海马CA1区神经元顶端树突的长度和树突的密度；40 mg·kg^-1反式白藜芦醇能增加AD小鼠海马CA1区BDNF、pCREB以及c-fos蛋白的表达。结论 反式白藜芦醇能逆转Aβ引起的小鼠的学习记忆损伤，其机制可能与改善海马神经元的神经可塑性有关。

Abstract:

OBJECTIVE To explore the effect of trans-resveratrol on learning and memory abilities in Alzheimer's disease(AD) mice induced by Aβ_25-35. METHODS The mice were randomly divided into 5 groups: sham operation group, model group and different doses groups of trans-resveratrol (10, 20, 40 mg·kg^-1). After injected with Aβ_25-35 into hippocampal CA1, the mice were administrated with trans-resveratrol for 10 d. The morris water maze was used to affirm the effect of trans-resveratrol on the learning and memory abilities of AD mice. The immunohistochemical staining was used to observe neuronal plasticity changes in hippocampal CA1 region. The expression of neural plasticity-related proteins was measured by western-blot. RESULTS The 40 mg·kg^-1 trans-resveratrol significantly decreased the escape latency in hidden platform test and increased the time spent in the platform quadrant and the number of platform location crossings. The 20, 40 mg·kg^-1 trans-resveratrol increased apical neurons dendritic length and apical dendritic points in hippocampal CA1 region. The 40 mg·kg^-1 trans-resveratrol increased the expression of neural plasticity-related proteins (BDNF, pCREB and c-fos protein) in hippocampal CA1 region of AD mice. CONCLUSION Trans-resveratrol reverses Aβ-induced learning and memory damage in mice, and the mechanism may relate to neural plasticity in hippocampal region.