黄芪对注射性坐骨神经损伤致大鼠肌萎缩的改善作用

Improvement of Huangqi Injection on Muscle Atrophy Induced by Sciatic Nerve Injection Injury in Rats

关键词:  黄芪  注射性坐骨神经损伤  胶原纤维  肌萎缩

Key words:  Huangqi  sciatic nerve injection injury  collagen fibers  muscular atrophy

摘要:

目的 探索黄芪改善注射性坐骨神经损伤大鼠肌萎缩的机制。方法 SD大鼠24只，♀♂不拘，随机分为4组：正常组、模型组、阴性对照组和黄芪干预组。采用天平称量肌重，HE染色后Leica图像分析仪测量肌纤维横切面积，VG染色法观察腓骨长肌内胶原纤维的变化。结果 青霉素注射坐骨神经后2周，与正常组相比，模型组大鼠跛行，腓骨长肌肌块缩小，色泽变暗，肌重减轻，肌纤维横切面积减小，胶原纤维明显增生；阴性对照组进一步加重；黄芪干预组上述变化明显好转。结论 黄芪能通过减少大鼠腓骨长肌胶原纤维增生，改善青霉素注射坐骨神经损伤引起的肌萎缩。

Abstract:

OBJECTIVE To explore the mechanisms that Huangqi injection improve the muscle atrophy induced by sciatic nerve injection injury in rats. METHODS Twenty four SD rats, either ♀ or ♂, were randomly divided into four groups named the normal group, the model group, the negative control group, and the Huangqi injection group. Muscle weights were weighed by scales, the cross-sectional area of muscle fibers were measured under Leica image analyzer after HE staining, the collagen fibers in musculi peronaeus longus were observed by VG staining. RESULTS After sciatic nerve penicillin injection 2 weeks, compared with the normal group, the model group rats walked with a limp, musculi peronaeus longus shrank and lightened, muscle color darkened, cross-sectional area of muscle fibers decreased, proliferation of collagen fibers was obvious. However, the above changes were far worse in the negative control group and significantly improved in the Huangqi injection group. CONCLUSION Huangqi injection can improve sciatic nerve penicillin injection-induced muscle atrophy by decreasing the hyperplasia of collagen fiber in peroneus longus muscle in SD rats.