HPLC波长转换法同时测定小儿咳喘灵颗粒中(R,S)-告依春、绿原酸和甘草苷的含量

Simultaneous Determination of Epigoitrin, Chlorogenic Acid and Liquiritin in Child Kechuanling Granules by Wavelength Conversion HPLC

关键词:  高效液相色谱  小儿咳喘灵颗粒  (R,S)-告依春  绿原酸  甘草苷

Key words:  HPLC  Child Kechuanling granules  epigoitrin  chlorogenic acid  liquiritin

摘要:

目的 建立高效液相色谱波长转换法同时测定小儿咳喘灵颗粒中(R,S)-告依春、绿原酸和甘草苷含量的方法。方法 样品采用70%甲醇溶液超声提取，采用Inertsil ODS-SP C18色谱柱(4.6 mm×250 mm，5 μm)分离；流动相：0.1%磷酸-乙腈，梯度洗脱；流速为1.0 mL·min^-1；检测波长：0~13 min为245 nm，13~50 min为217 nm。结果 (R,S)-告依春、绿原酸和甘草苷分别在0.700~14.000 μg·mL^-1、4.780~95.600 μg·mL^-1、1.050~21.008 μg·mL^-1内具有良好的线性关系；加样回收率分别为98.8%，102.8%和97.7%；RSD分别为1.2%，1.9%和1.4%(n=9)。结论 该方法简单易行，准确性和重复性好，可用于该制剂的质量控制。

Abstract:

OBJECTIVE To determine the content of epigoitrin, chlorogenic acid and liquiritin in child kechuanling granules by wavelength conversion HPLC. METHODS The sample was extracted by 70% methanol and separated on a Inertsil ODS-SP C₁₈(4.6 mm×250 mm, 5 μm). The mobile phase were 0.1% phosphoric acid and acetonitrile, gradient elution. The flow rate was 1.0 mL·min^-1 while the ultraviolet detector was operated at 245 nm(0-13 min) and 217 nm(13-52 min). RESULTS The linear range of epigoitrin, chlorogenic acid and liquiritin was respectively in 0.700-14.000 μg·mL^-1, 4.780-95.600 μg·mL^-1, 1.050-21.008 μg·mL^-1 while the average recovery was 98.8%, 102.8%, 97.7% with the RSD 1.2%, 1.9%, 1.4%(n=9). CONCLUSION This method is simple, rapid and with good repeatability, so it could be used in quality control of child kechuanling granules.