柳茶提取物对异烟肼引起的肝损伤小鼠CYP2E1酶活性及mRNA表达的影响

Effect of Extracts of Sibraea Angustata (Rchd.) Hand-Mazz. on the Enzyme Activity and mRNA Expression of CYP2E1 in Mice with Isoniazid-induced Liver Injury

关键词:  柳茶提取物  肝损伤  异烟肼  CYP2E1

Key words:  extracts of Sibraea angustata (Rchd.) Hand-Mazz.  liver injury  isoniazid  CYP2E1

摘要:

目的 研究柳茶提取物对异烟肼引起小鼠肝损伤的CYP2E1基因表达的影响。方法 60只小鼠随机分为正常对照组、模型对照组、阳性对照组(水飞蓟宾胶囊)和柳茶提取物低、中、高剂量组(1.0，1.5，2.0 g·kg^-1)，分别灌胃给予相应的药物，连续10 d，检测各组血清(ALT、AST)及肝脏相关生化指标以及肝脏组织(MDA、SOD、GSH-PX）、病理学变化、CYP2E1酶活性及mRNA表达。结果 与模型组比较，柳茶提取物组血清及肝脏相关生化指标有显著改善(P<0.05或0.001)，病理学研究进一步证明了其保护作用，CYP2E1的酶活性及mRNA表达均明显降低(P<0.05)。结论 柳茶提取物可能通过抑制CYP2E1的活性和表达来减少自由基的产生，从而达到减轻异烟肼引起的小鼠氧化性肝损伤作用。

Abstract:

OBJECTIVE To explore the effect of the extracts of Sibraea angustata (ESA) on gene expression of CYP2E1 in mice with liver injury induced by isoniazid(INH). METHODS Sixty mice were randomly divided into normal control group, model control group, positive control group (Silybin capsules) and three doses(1.0, 1.5, 2.0 g·kg^-1) groups of ESA. After intragastric administrated with corresponding drugs for 10 days, the biochemical indicators in surum(AST, ALT) and liver (MDA, SOD, GSH-PX), histological patterns, the activity and mRNA epression of CYP2E1 in mice were measured and compared. RESULTS Compared with model control group, the activity of related biochemical indexs ameliorated significantiy(P<0.05 or 0.001), which were confirmed by histopathological analysis. The enzyme activity and mRNA expression of CYP2E1 decreased significantly in ESA-treated groups (P<0.05). CONCLUSION ESA may exerts its hepatoprotective activity against INH-induced liver injury by inhibiting the activity and expression of CYP2E1 andproducyion free radical.