HPLC同时测定野菊花中绿原酸、咖啡酸和蒙花苷的含量

Simultaneous Determination of Chlorogenic Acid, Caffeic Acid and Linarin in Chrysanthemi Indici Flos by HPLC

关键词:  野菊花  绿原酸  咖啡酸  蒙花苷  高效液相色谱法

Key words:  Chrysanthemi Indici Flos  chlorogenic acid  caffeic acid  linarin  HPLC

摘要:

目的 建立同时测定野菊花中绿原酸、咖啡酸和蒙花苷含量的方法。方法 采用高效液相色谱法，应用Agilent Eclipse C₁₈柱(250 mm×4.6 mm，5 μm)，二极管阵列检测器(DAD)，以甲醇-乙腈-0.2%乙酸溶液为流动相，梯度洗脱，流速为1 mL·min^-1，柱温为30 ℃，检测波长为334 nm。结果 绿原酸、咖啡酸和蒙花苷的峰面积与浓度的线性关系良好(r>0.999 3)，平均加样回收率分别为99.5%，100.4%，100.4%。结论　该方法简单、准确、重复性好，可为全面评价和控制野菊花的质量提供依据。

Abstract:

OBJECTIVE To simultaneous determine the content of chlorogenic acid, caffeic acid and linarin in Chrysanthemi Indici Flos. METHODS The HPLC method with DAD was used in the assay. All separations were carried out on an Agilent Eclipse C₁₈ column (250 mm×4.6 mm, 5.0 μm). A linear gradient elution of methanol-acetonitrile-0.2% aqueous acetic acid was used to run the separation. The solvent flow rate was 1.0 mL·min^-1 and the column temperature was maintained at 30 ℃. The detection wavelength was 334 nm. RESULTS Excellent linear behavior over the investigated concentration range was observed, with r>0.999 3 for all the analytes. The average recovery values of chlorogenic acid, caffeic acid and linarin were 99.5%, 100.4%, 100.4%, respectively. CONCLUSION The method is simple, accurate and reproducible, contributing to quality evaluation and control of Chrysanthemi Indici Flos.