乌药对小鼠免疫功能及大鼠实验性脂肪肝的影响

Effects of Linderae Radix on the Immunological Function of Mice and Experimental Fatty Liver of Rats

关键词:  乌药  免疫功能  实验性脂肪肝  护肝作用

Key words:  Linderae Radix  immunological function  fatty liver  hepatoprotective effect

摘要:

目的 探讨乌药对小鼠免疫功能及大鼠实验性脂肪肝的影响。方法 乌药提取液以生药4，2，1 g·kg^-1连续灌胃给予小鼠30 d，测定给药后小鼠的脾脏指数、胸腺指数、对Con A诱导的淋巴细胞增殖能力及抗体生成细胞数；以生药2，1，0.5 g·kg^-1灌胃给予高脂饲料致实验性脂肪肝的SD大鼠，连续给药21 d后测定大鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、谷丙转氨酶(ALT)和谷草转氨酶(AST)含量。结果 与对照组比较，乌药4和2 g·kg^-1均能显著提高胸腺指数和Con A诱导的淋巴细胞增殖能力及抗体生成细胞数；4和1 g·kg^-1能显著增加脾脏指数；与模型组比较，乌药2 g·kg^-1能显著降低大鼠血清TC、LDL-C、ALT、AST水平，1 g·kg^-1能显著降低血清TC、AST水平。结论 乌药能提高小鼠免疫功能，对大鼠因喂饲高脂饲料致实验性脂肪肝有一定的降脂和护肝作用。

Abstract:

OBJECTIVE To observe the effects of Linderae Radix on the immunological function of mice and experimental fatty liver of rats. METHODS ICR mice were orally administered with 4, 2 and 1 g·kg^-1 dosage of Linderae Radix respectively. The organ index of spleen and thymus, T lymphocyte transformation ability, the numbers of antibody producing cells were observed at the 30th day. SD rats modeled with taking high-fat diet were orally administered with 2, 1 and 0.5 g·kg^-1 dosage of Linderae Radix respectively for 21 d. Serum levels of TC, triglyceride(TG), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C), alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were detected at the 21st day. RESULTS Compared with the control group, 4, 2 g·kg^-1 Linderae Radix could raise the organ index of thymus, enhance the ability of T lymphocyte transformation and increase the number of antibody-producing cells. The 4, 1 g·kg^-1 dosage could raise the organ index of thymus. Compared with the model group, 2 g·kg^-1 of Linderae Radix could reduce the serum level of TC, LDL-C, AST, ALT of the model rats, 1 g·kg^-1 dosage could reduce the serum level of TC, AST of the model rats. CONCLUSION Linderae Radix can enhance the immunological function of mice and has hepatoprotective effect on experimental fatty liver of rats.