大蒜油对实验小鼠的镇咳祛痰作用

Antitussive and Expectorant Effects of Garlic Oil on Experimental Mice

关键词:  大蒜油  镇咳  祛痰

Key words:  garlic oil  antitussive  expectorant

摘要:

目的 探讨大蒜油对小鼠的镇咳祛痰作用。方法 以玉米油作为溶剂溶解大蒜油，将大蒜油分高(100 mg?kg^-1)、中(50 mg?kg^-1)、低(25 mg?kg^-1)3种浓度灌胃小鼠后，采用氨水引咳法观察大蒜油镇咳作用，同时取气管进行病理组织学检查，并以氢溴酸右美沙芬糖浆(10 mL?kg^-1)作阳性对照。采用酚红气管排泌法观察大蒜油祛痰作用，并以盐酸氨溴索口服溶液(4 mL?kg^-1)作阳性对照。结果 大蒜油的镇咳、祛痰作用与溶剂对照组比较有显著的差异性(P<0.05或P<0.01)，并存在剂量效应关系；大蒜油还可抑制气管黏膜炎症、充血及黏液分泌，其综合药理作用优于氢溴酸右美沙芬糖浆。结论 大蒜油具有显著的镇咳祛痰及黏膜保护作用。

Abstract:

OBJECTIVE To investigate the antitussive and expectorant effect of garlic oil on experimental mice. METHODS Corn oil was used to dilute garlic oil, and intragastric administration high(100 mg?kg^-1), middle(50 mg?kg^-1) and low(25 mg?kg^-1) doses of garlic oil on mice. Then antitussive test was observed on mice with cough induced by ammonia water, and tissue slicing and HE staining were made to observe structure changes of tracheal tissue in each group. A phenol red excretion test was used to investigate the expectorant effect of garlic oil in mice. Dextromethorphan hydrobromide(10 mL?kg^-1) and ambroxol hydrochloride syrup 4 mL?kg^-1 were adopted for positive drug control. RESULTS Garlic oil was significantly effective on mice with cough(P<0.05 or 0.01). There was an obvious dose-response relationship in the antitussive and expectorant effects of garlic oil in comparison with model group. In addition, garlic oil inhibited inflammatory reaction, congestion and mucous secretion in tunica mucosa tracheae. Moreover, garlic oil had better pharmacological actions than dextromethorphan hydrobromide. CONCLUSION Garlic oil has significant antitussive, expectorant and tracheal mucosal protection efficacy.