HPLC测定三七提取液中三七皂苷R2(S)、七叶胆苷XVII和人参皂苷F2的含量

Determination of Notoginsenoside R2(S), Gypenoside XVII and Ginsenoside F2 in Notoginseng Radix et Rhizoma Extracts by HPLC

关键词:  三七提取液  三七皂苷R₂(S)  七叶胆苷XVII  人参皂苷F₂  高效液相色谱法

Key words:  Panax notoginseng extracts  notoginsenoside R₂(S)  gypenoside XVII  ginsenoside F₂  HPLC

摘要:

目的 建立同时测定三七提取液中三七皂苷R₂(S)、七叶胆苷XVII及人参皂苷F₂含量的高效液相色谱法。方法 色谱柱为Waters Acquity UPLC CSH-C₁₈(50 mm×2.1 mm，1.7 μm)，流动相为0.01%甲酸-水(A)和0.01%甲酸-乙腈(B)，梯度洗脱，检测波长为203 nm，进样量为5 μL，流速为0.35 mL·min^-1，柱温为40 ℃。结果 在43 min内可完成三七皂苷R₂(S)、七叶胆苷XVII和人参皂苷F₂的分离测定。3种皂苷峰面积和浓度线性关系良好(r²>0.999 8)；日内和日间精密度RSD<3.4%；回收率为98.4%~102.1%。结论 该方法简便、准确，重复性好，可用于三七提取液中皂苷成分的测定。

Abstract:

OBJECTIVE To develope an HPLC method for the simultaneous determination of notoginsenoside R₂(S), gypenoside XVII and ginsenoside F₂ in Notoginseng Radix et Rhizoma extracts. METHODS The analysis was performed on an Agilent 1260 series HPLC system with Waters Acquity UPLC CSH-C₁₈ (50 mm×2.1 mm, 1.7 μm) column. Gradient elution using 0.01%-formic acid-water and 0.01%-formic acid-acetonitrile as the mobile phases with detection wavelength of 203 nm, flow rate of 0.35 mL·min^-1, injection volume 5 μL and column temperature 40 ℃ was applied. RESULTS The total analyzing time was 43 min. The high determination coefficient values (r²>0.999 8) indicated good correlations between saponin concentrations and their peak areas within the test ranges. The overall intra-day and inter-day variations (RSD) of 3 major saponins were <3.4%. The developed method was successfully used for the analysis of saponins in Notoginseng Radix et Rhizoma extracts with overall recovery of 98.4%–102.1% for all the analytes. CONCLUSION This analytical method is accurate and convenient for the determination of 3 saponins in Panax notoginseng extracts.