HPLC同时测定鱼腥草芩蓝合剂中3种活性成分的含量

Simultaneous Determination of Three Ingredients in Yuxingcao Qinlan Mixtures by HPLC

关键词:  高效液相色谱法  绿原酸  黄芩苷  黄芩素

Key words:  HPLC  chlorogenic acid  baicalin  baicalein

摘要:

目的 建立HPLC测定鱼腥草芩蓝合剂中绿原酸、黄芩苷和黄芩素的含量。方法 采用Kromasil-100 C₁₈色谱柱(4.6?mm×250 mm，5 μm)，流动相为甲醇-0.2%磷酸梯度洗脱，流速为1.0 mL·min^-1，检测波长为322 nm，柱温为35 ℃。结果 绿原酸、黄芩苷和黄芩素线性范围分别为0.022 4~1.12 μg(R²=0.999 7)，0.045 8~2.29 μg(R²=0.999 5)和0.031 8~1.59 μg(R²=0.999 9)；3种成分的平均回收率分别为99.45%(RSD=1.4%)，99.37%(RSD=2.0%)和97.90%(RSD=1.7%)。结论 本方法快速简便，精密度高，稳定性和重复性好，可用于鱼腥草芩蓝合剂中绿原酸、黄芩苷和黄芩素3种成分的定量分析和药品质量控制。

Abstract:

OBJECTIVE To establish an HPLC method for the simultaneous determination of chlorogenic acid, baicalin and baicalein in Yuxingcao qinlan mixtures. METHODS HPLC conditions included Kromasil-100 C₁₈ (250 mm×4.6 mm, 5 μm) and the mobile phase was a mixture of methanol and 0.1% phosphoric acid for gradient elution. The flow rate was 1.0 mL·min^-1. The wavelength was 322 nm and column temperature was 35 ℃. RESULTS Three ingredients had good linear relationships: in the range of 0.022 4-1.12 μg(R²=0.999 7), baicalin in the range of 0.045 8-2.29 μg(R²=0.999 5) and baicalein in the range of 0.031?8-1.59 μg(R²=0.999 9). Their average recoveries were 99.45%(RSD=1.4%), 99.37%(RSD=2.0%) and 97.90%(RSD=1.7%), respectively. CONCLUSION This method was simple with high precision, good repeatability and stability. It could be used for the determination of the three ingredients and quality control of Yuxingcao qinlan mixtures.