浙江七叶一枝花种质资源的化学评价

Chemical Evaluation of Germplasm Resources of Paris Polyphylla Smith Var. Chinensis (Franch.) Hara in Zhejiang Province

关键词:  七叶一枝花  种质资源  化学评价  家化栽培

Key words:  Paris polyphylla Smith var. chinensis (Franch.) Hara  germplasm recourses  chemical evaluation  domestic cultivation

摘要:

目的 测定浙江不同采集地野外七叶一枝花中主要活性成分甾体皂苷的含量，为筛选优质种质资源和适宜栽培地提供依据。方法 采用高效液相色谱法，色谱柱为Waters XBridge C₁₈柱(4.6 mm×250 mm，5 μm)；流动相为乙腈(A)-水(B)，梯度洗脱，柱温：25 ℃，洗脱流速为1.0 mL·min^-1，测定12个不同采集地七叶一枝花中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的含量。结果 来自不同采集地的七叶一枝花种源间的重楼皂苷含量存在显著差异，有一半种源的皂苷含量达不到药典规定的标准，优质种源来自永嘉上桥头村，4种重楼皂苷之和为2.928%，明显高于其他种源。结论 浙南山区较适合七叶一枝花的人工种植。

Abstract:

OBJECTIVE To screen fine germplasm resources and suitable cultivated place for Paris polyphylla Smith var. chinensis (Franch.) Hara by determining the content of 4 main active steroidal saponins collected from different habitats. METHODS HPLC method was applied to determine the content of steroidal saponins Ⅰ, Ⅱ, Ⅵ, Ⅶ in 12 wild germplasm resources of Paris polyphylla Smith var. chinensis (Franch.) Hara. The column was Waters XBridge C₁₈(250 mm×4.6 mm, 5 μm ). The mobil phase was acetonitrile(A)-water(B). Gradient elution was used. The column temperature was 25 ℃ and the flow rate was 1.0 mL·min^-1. RESULTS Significant differences existed in the polyphyllin content of Paris polyphylla Smith var. chinensis (Franch.) Hara samples collected from different habitats. The polyphyllin content of half tested samples could not reach the criteria of Chinese Pharmacopoeia. The high quality Paris polyphylla Smith var. chinensis (Franch.) Hara was selected from Yongjia, Wenzhou with four polyphyllins total content of 2.928%, significantly higher than those of other habitats. CONCLUSION The mountainous areas of south Zhejiang are suitable for the cultivation of Paris polyphylla Smith var. chinensis (Franch.) Hara.