土三七对大鼠血液白细胞及分类计数、ET、NO、TNF-α和IL-1β的影响

Effect of Gynura Segetum Merr. on the Counts of White Blood Cell, Its Classification and the Content of ET, NO, TNF-α and IL-1β in Rats

关键词:  土三七  肝小静脉闭塞  白细胞  内皮素  一氧化氮  肿瘤坏死因子-α  白介素-1β

Key words:  Gynura segetum Merr.  hepatic venular occlusive disease  white blood cells  endothelin  nitric oxide  tumor necrosis factor-α  interleukin-1β

摘要:

目的 观察土三七对正常大鼠血液中白细胞及分类计数、内皮素(endothelin，ET)、一氧化氮(nitric oxide，NO)、肿瘤坏死因子-α(tumor necrosis factor，TNF-α)、白介素-1β(interleukin，IL-1β)含量的影响。方法 取60只SD大鼠，♀♂各半，分为空白对照组、三七对照组(10 g·kg-1)和土三七低、中、高剂量组(5，10，20 g·kg-1)，连续给药4周，观察土三七对大鼠白细胞分类、血管内皮分泌功能(NO、ET)、炎症因子应答(TNF-α、IL-1β)、脾脏和组织病理的变化。结果 与空白对照组比较，给药2周，土三七各剂量组白细胞数、中性粒细胞数、淋巴细胞数、单核细胞数和嗜碱粒细胞数及TNF-α、IL-1β含量均显著升高(P<0.01)，低剂量组ET含量显著降低。给药4周，土三七中剂量组大鼠的白细胞数、中性粒细胞数、淋巴细胞数和单核细胞数显著升高(P<0.05或0.01)，低、高剂量组白细胞数无明显差异；各剂量组TNF-α和IL-1β含量显著升高(P<0.01)，低、中剂量组ET含量显著降低(P<0.05或0.01)，高剂量组ET、NO水平均显著升高(P<0.01)；高剂量组脾脏中脾小结明显减少，未见生发中心，大量淋巴细胞弥漫分布，红髓血窦减少，肝脏大量充血。结论 土三七对大鼠的损害可能以炎症为主，土三七所致肝小静脉闭塞是血管内皮的炎症肿胀引起狭窄，而非血栓形成；这对临床治疗该疾病有一定的指导意义。

Abstract:

OBJECTIVE To explore the anti-vitiligo effects of Kebai particles in vitro and in vivo and evaluate the safety of Kebai particles. METHODS The effects of Kebai particles on mouse melanoma cells B16f10 proliferation in vitro was tested by serum pharmacology method. Long term toxicity test were carried out to evaluate the safety of Kebai particles. 50 guinea pigs were randomly divided into 5 groups: normal control group, model control group, high, middle and low dose group of Kebai particles(9.04，4.52，2.26 g·kg^-1·d^-1). The experimental vitiligo guinea pig model was prepared by 7% H₂O₂ for 50 d. Melanin distribution in skin and cholinesterase(CHE), monoamine oxidase(MAO), malondialdehyde(MDA) and tyrosinase(TYR) in blood were determined. RESULTS The results of serum pharmacology experiment showed that medicated serum significantly promoted the proliferation of B16f10 cells in vitro. Long-term toxicity experiment results indicated Kebai particles exhibited no effect on viscera weight of SD rats. Animal experimental data showed that the serum level of MAO and MDA significantly increased in model control group(P<0.05), while the serum level of CHE and TRY significantly decreased in model control group(P<0.01 or 0.05). Compared with the model control group, high dose of Kebai particles significantly decreased the serum content of MDA(P<0.01), high and middle dose of Kebai particles significantly decreased the content of MAO and increased the content of CHE(P<0.05). CONCLUSION Kebai particles show good anti-vitiligo effects, its main mechanism may be related to decrease the contents of serum CHE, MAO and MDA and promote the proliferation of melanocytes.