骨碎补总黄酮对骨质疏松模型大鼠OPG/RANKL/RANK轴系统的影响

Effect on OPG/RANKL/RANK Axial System in Osteoporotic Rats by Total Flavones of Drynariae Rhizoma

关键词:  骨质疏松  骨碎补总黄酮  雌二醇  OPG/RANKL/RANK轴系统

Key words:  osteoporosis  total flavones of Drynariae Rhizoma  estrogen  OPG/RANKL/RANK axial system

摘要:

目的 研究不同剂量骨碎补总黄酮灌胃对骨质疏松模型大鼠雌激素水平及骨保护素(orthopantomography，OPG)、核因子κB受体活化因子(receptor activator of NF-κB，RANK)和核因子κB受体活化因子配体(receptor activator of NF-κB ligand，RANKL)表达的影响，探讨其对骨代谢影响的可能机制。方法 通过去卵巢法造成骨质疏松大鼠模型，以戊酸雌二醇片0.1 mg·kg^-1及低、中、高不同剂量骨碎补总黄酮(0.054，0.108，0.216 g·kg^-1·d^-1)喂养3个月后，取动脉血，采用放射免疫法测定雌激素水平，用酶联免疫吸附法(ELISA)检测破骨细胞OPG、RANK和RANKL的表达。结果 与模型组2相比，各给药组雌二醇及OPG的表达量增加(P<0.05)，RANK和RANKL的表达量减少(P<0.05)，且随着骨碎补总黄酮剂量的增加，雌二醇及OPG表达呈上升趋势，RANK和RANKL表达均呈下降趋势。结论 不同剂量骨碎补总黄酮均影响OPG/RANKL/RANK轴系统，且随骨碎补总黄酮剂量的增加效果越明显，可能是通过调控OPG/RANKL/RANK轴系统使OPG表达增加、RANK和RANKL的表达下降来实现的。

Abstract:

OBJECTIVE To investigate the effect of different doses of total flavones of Drynariae Rhizoma on the expression of estrogen and orthopantomography(OPG), receptor activator of NF-κB(RANKL), receptor activator of NF-κB ligand(RANK) in osteoporotic rats through administration by gavage, and explore the effect of total flavones of Drynariae Rhizoma on the bone metabolism in osteoporotic rats model. METHODS osteoporotic rats model were established by cutting out their ovaries, were given estradiol (0.1 mg·kg^-1) and low, middle, high dose (0.054, 0.108, 0.216 g·kg^-1·d^-1) of total flavones of Drynariae Rhizoma by gavage for 3 months. Then draw their arterial blood. the changes of estrogen level were observed by radioimmunoassay and OPG, RANKL, RANK level by ELISA. RESULTS Campared with model control 2 group, the estrogen and OPG content were obviously increased (P<0.05), the RANKL and RANK content were significantly decreased (P<0.05). At the same time, the estrogen and OPG rose along with the dose of total flavones of Drynariae Rhizoma while the RANKL and RANK dropped. CONCLUSION different doses of total flavones of Drynariae Rhizoma can all affect OPG/RANKL/RANK axial system, and the effect rise along with the concentration of total flavones of Drynariae Rhizoma, which may be realized by increasing the OPG level and decreasing the RANKL and RANK level.