基于近红外光谱的疏血通注射液浸提过程总固体含量分析

Quantitative Analysis of Total Solid Content in Lixivating Process of Shuxuetong Injection Using Near-infrared Spectroscopy

关键词:  近红外光谱  疏血通注射液  浸提过程  总固体含量

Key words:  near infrared spectroscopy  Shuxuetong injection  lixivating process  total solid content

摘要:

目的 建立一种快速测定疏血通注射液浸提过程中总固体含量的近红外光谱法。方法 以水蛭和地龙2种药材浸提过程为例，采用偏最小二乘法(PLS)建立总固体含量的近红外光谱分析校正模型，实现对总固体含量的快速测定。结果 近红外光谱在一阶导数结合Karl Norris平滑滤波处理下，建模效果最佳。水蛭总固体含量校正集相关系数(R)为0.810 8，校正集和验证集预测误差均方根(RMSEC、RMSEP)分别为0.583和0.495，交叉验证误差均方根(RMSECV)为0.81，校正集和验证集相对偏差(RSEC、RSEP)分别为6.11%和5.25%；地龙总固体含量校正集R值为0.975 5，RMSEC和RMSEP分别为1.10和1.85，RMSECV为1.61，RSEC和RSEP分别为4.68%和7.80%。结论 近红外光谱可用于快速测定疏血通注射液浸提过程中总固体含量，有望推广应用于中药浸提过程的在线质量控制。

Abstract:

OBJECTIVE To develop a method for total solid content determination of Shuxuetong injection in lixivating process based on near-infrared spectroscopy. METHODS Taking the lixivating process of Hirudo and Earthworm for examples, the quantitative calibration models for the total solid content were established by partial least squares (PLS). And the established PLS calibration models were used to rapidly analyze the total solid content in lixivating process of Shuexuetong injection. RESULTS Optimal models were obtained when raw spectra were processed with first derivative combined with Karl Norris smoothing. The correlation coefficient of total solid content was 0.810 8 for lixivating process of Hirudo. Root mean square error of calibration and prediction were 0.583 and 0.495, respectively. Root mean square error of cross validation was 0.81, and relative standard errors of calibration and prediction was 6.11% and 5.25%, respectively. The correlation coefficient of total solid content was 0.975 5 for lixivating process of Earthworm. Root mean square error of calibration and prediction were 1.10 and 1.85, respectively. Root mean square error of cross validation was 1.61, and relative standard errors of calibration and prediction was 4.68% and 7.80%, respectively. CONCLUSION NIR spectroscopy was successfully applied to determine total solid content in lixivating process of Hirudo and Earthworm. It can be used for the quality control in manufacturing process of Shuxuetong Injection.