通络汤对腰椎间盘突出症模型大鼠的影响

Effect of Tongluo Decoction on Luber Intervertebral Disc Herniation in Rats

关键词:  腰椎间盘突出症  通络汤  治疗作用

Key words:  lumbar intervertebral disc herniatio  Tongluo decoction  curative effect

摘要:

目的 探讨通络汤对腰椎间盘突出症模型大鼠的治疗作用。方法 48只大鼠随机分为6组：正常组、模型组、阳性对照组、通络汤高、中、低剂量组，除正常组外，各组均通过手术建立大鼠自体尾髓核移植所致机械性压迫髓核突出动物模型。热板法测定痛阈，玻片法测定凝血时间，血液流变仪测定血液流变学指标，酶免法检测肿瘤坏死因子-α(TNF-α)、前列腺素E2(PGE2)、一氧化氮(NO)含量和一氧化氮合成酶(NOS)活性；取神经根进行病理学检测。结果 大鼠腰突症模型建立后痛阈降低，凝血时间缩短，血液黏度增大，血清NO、NOS、TNF-α、PGE2含量升高，神经根组织出现炎性浸润。通络汤可显著改善这些症状，表现为提高痛阈，延长凝血时间，降低血液黏度，抑制血清中炎症因子NO、NOS、TNF-α、PGE₂的产生，减少神经根组织炎性浸润的产生。结论 通络汤能够有效缓解腰椎间盘突出症疼痛症状，这可能与减轻神经根组织的充血、水肿、炎症反应，降低全血黏度、血浆黏度，延长凝血时间，改善血液循环等有关。

Abstract:

OBJECTIVE To explore the curative effect of Tongluo decoction on luber intervertebral disc herniation(LIDH) in rats. METHODS The 48 rats were randomly divided into 6 groups: normal group, model group, positive control group, Tongluo decoction high, middle and low dose groups. Non-compressive animal model of lumbar nucleus pulposus prolapse were established by operation. Pain threshold was determined by hot plate method. Clotting time was detected by slide method. Changes of hemorheology were detected by hemorheometer. The level of tumor necrosis factor-α(TNF-α), prostaglandins 2(PGE₂) and nitric oxide(NO), nitric oxide synthase(NOS) were measured by enzyme linked immunosorbent assay. Pathological changes of nerve root were observed by pathological detection. RESULTS In rats with LIDH, the pain threshold decreased; clotting time shorten, blood viscosity increased; the level of inflammatory cytokines such as NO, NOS, TNF-α and PGE₂ rised, and the inflammatory infiltrates were observed in nerve root tissue. Tongluo decoction could ameliorate those symptoms, presented as increasing pain threshold; prolonging clotting time; lowering blood viscosity; inhibiting inflammatory cytokines(NO, NOS, TNF-α and PGE₂) in serum and reducing the inflammatory cell infiltration in the nerve root tissue. CONCLUSION The curative effect of Tongluo decoction on LIDH in rats may be associated with its ability of anti-inflammatory, analgesic action; improve blood circulation, and degrade the level of inflammatory factors(NO, NOS, TNF-α and PGE₂).