| 引用本文: | 任红,乔洪翔,方玲,王如伟.HPLC-ELSD测定银杏叶多糖组成及单糖含量[J].中国现代应用药学,2015,32(6):711-714. |
| REN Hong,QIAO Hongxiang,FANG Ling,WANG Ruwei.Determination of Polysaccharides Composition and Monosaccharide Content in Ginkgo Folium by HPLC-ELSD[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(6):711-714. |
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| 摘要: |
| 目的 建立高效液相色谱法测定银杏叶多糖组成及单糖含量。方法 采用Agilent Zorbax Carbohydrate色谱柱(250?mm×4.6 mm，5 μm)，乙腈-水(80∶20)为流动相，流速：1.0 mL·min-1，柱温：30 ℃。结果 色谱峰分离良好，鼠李糖在4.160~20.80 μg、阿拉伯糖在8.040~40.20 μg、果糖在4.096~20.48 μg、甘露糖在4.000~20.00 μg、葡萄糖在8.152~ 40.76?μg、半乳糖在3.960~19.80 μg内呈良好的线性关系。平均加样回收率鼠李糖为99.6%、阿拉伯糖为100.9%、果糖为99.7%、甘露糖为99.1%、葡萄糖为98.8%、半乳糖为100.1%。结论 该方法简单快速、稳定可行，可用于银杏叶中多糖成分质量控制。 |
| 关键词: 银杏叶 多糖 单糖 含量测定 质量控制 |
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| 基金项目:“重大新药创制”科技重大专项(2011ZX09203-001-18)；浙江省科技计划项目(2009E10009) |
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| Determination of Polysaccharides Composition and Monosaccharide Content in Ginkgo Folium by HPLC-ELSD |
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REN Hong1, QIAO Hongxiang2,3, FANG Ling3, WANG Ruwei1,4
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1.Pharmacy College, Zhejiang Chinese Medicine University, Hangzhou 310053, China;2.Yunnan Institute of Botony of Conba Group, Kunming 650217, China;3.The key Laboratory of Chinese Medicine Pharmaceutical Technology in Zhejiang Province, Hangzhou 310051, China;4.Zhejiang Modern Traditional Chinese Medicine and Natural Medicine Research Institute Co., Ltd., 310051, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for determination of polysaccharides composition and the monosaccharide content from Ginkgo Folium. METHODS The analysis was performed on Agilent Zorbax Carbohydrate (250?mm×4.6 mm, 5 μm) chromatographic column, and the mobile phase was acetonitrile-water(80∶20), the flow rate was 1.0?mL·min-1 and the column temperature was maintained at 30 ℃. RESULTS The calibration curves were linear within the range of 4.160-20.80 μg for rhamnose, 8.040-40.20 μg for arabian sugar, 4.096-20.48 μg for fructose, 4.000-20.00 μg for mannose, 8.152-40.76 μg for glucose and 3.96-19.8 μg for galactose respectively. The recoveries of rhamnose, arabian sugar, fructose, mannose, glucose, galactose were 99.6%, 100.9%, 99.7%, 99.1%, 98.8%, 100.1%, respectively. CONCLUSION This method is simple and rapid, stability and feasible, it can be used for the quality control of polysaccharide compositions in ginkgo biloba leaves. |
| Key words: Ginkgo Folium polysaccharides simple sugars content determination quality control |
