蚕虫草不同提取物止咳化痰作用研究

Antitussive and Expectorant Effects of Different Extracts of Cordyceps Militaris(L.) Link.

关键词:  蚕虫草  止咳  化痰

Key words:  Cordyceps militaris(L.) Link.  antitussive  expectorant

摘要:

目的 研究蚕虫草水提取物和70%、95%乙醇提取物的止咳、祛痰作用。方法 采用氨水喷雾法造模，记录小鼠咳嗽潜伏期以及3 min 内的咳嗽次数；采用枸橼酸喷雾法造模，记录豚鼠咳嗽潜伏期以及5 min内的咳嗽次数；采用酚红气管排泌法观察祛痰作用。结果 与对照组比较，蚕虫草水提取物和70%乙醇提取物高中剂量、95%乙醇提取物高剂量均可显著延长咳嗽潜伏期、减少咳嗽次数、增加酚红排泌量；水提取物低剂量显著延长咳嗽潜伏期和咳嗽次数，对酚红排泌量无显著影响；70%乙醇提取物低剂量对上述指标均无明显影响；95%乙醇提取物中低剂量对咳嗽潜伏期和咳嗽次数组均无明显影响，但可明显增加酚红排泌量。结论 蚕虫草具有明显止咳祛痰作用，水溶性成分作用强于醇溶性成分。

Abstract:

OBJECTIVE To explore the antitussive and expectorant effects of water and 70%, 95% ethanol extracts of Cordyceps militaris(L.) Link.. METHODS The incubative period and the times of cough in 3 min of mice were observed after sprayed by ammonia. The incubative period and the times of cough in 5 min of guinea pigs were observed after sprayed by citric acid. A phenol red excretion test was used to investigate the expectorant effect of the different extracts of Cordyceps militaris(L.) Link. in mice. RESULTS Compared with control group, the high and low doses of water and 70% ethanol extracts significantly prolonged the incubative period and the excretion of phenolsulfonphthalein, and reduced the times of cough. The low dose of water extract increased the incubative period and reduced the times of cough, but had no significant effect on the excretion of phenolsulfonphthalein. The low dose of 70% ethanol extracts had no significant effect on the above indexes. The middle and low doses of 95% ethanol extracts had no significant effects on the incubative period and the times of cough, but significantly increased the excretion of phenolsulfonphthalein. CONCLUSION Cordyceps militaris (L.) Link. has significant antitussive and expectorant efficacy, and the efficacy of the water-soluble components is stronger than the alcohol-soluble components.