半枝莲粗多糖对S180荷瘤小鼠红细胞膜脂类成分的影响

Effect of Polysaccharides from Scutellaria Barbata D. Don on the Contents of Lipids of Erythrocyte Membrane in S180 Tumor-bearing Mice

关键词:  半枝莲粗多糖  抗肿瘤  红细胞膜磷脂  气相色谱-质谱联用

Key words:  polysaccharide from Scutellaria barbata D. Don  anti-tumor  phospholipid of erythrocyte membrane  GC-MS

摘要:

目的 探讨半枝莲粗多糖对S₁₈₀荷瘤小鼠红细胞膜脂类含量的影响。方法 48只小鼠，♂，随机分为正常对照组、模型对照组、阳性对照(黄芪多糖)组、半枝莲粗多糖低、中、高剂量(50，100，200 mg·kg^-1)组，除正常对照组外其他组小鼠右前腋皮下接种肿瘤。采用紫外分光光度计检测红细胞膜胆固醇、磷脂含量，采用GC-MS检测红细胞膜磷脂脂肪酸的百分含量。结果 与模型对照组比较，半枝莲粗多糖显著提高S₁₈₀荷瘤小鼠红细胞膜磷脂含量、降低胆固醇含量及二者比值，棕榈一烯酸(C_16∶1)、油酸(C_18∶1)、亚油酸(C_18∶2)、不饱和脂肪酸/总脂肪酸的比例均有不同程度的升高。结论 半枝莲粗多糖可增加S₁₈₀荷瘤小鼠红细胞膜磷脂含量，降低胆固醇及其比值，并且增加不饱和脂肪酸在总脂肪酸中的比例，可能通过这些指标共同作用提高红细胞膜的流动性，从而增加红细胞的免疫功能，实现抗肿瘤作用。

Abstract:

OBJECTIVE To explore the effects of polysaccharide from Scutellaria barbata D. Don on the contents of lipid in erythrocyte membrane in S₁₈₀ tumor-bearing mice. METHODS Forty-eight female mice were randomly divided into normal control group, model control group, positive control(astragalus polysaccharides) group, and low, middle, high dose(50, 100, 200 mg·kg^-1)group of polysaccharide from Scutellaria barbata D. Don. The mice in other groups except normal control group got subcutaneous tumor. UV spectrophotometer was used to measure the contents of cholesterol and phospholipid in erythrocyte membrane. The percentage composition of phospholipids fatty acid in erythrocyte membrane was measured by GC-MS. RESULTS Compared with the model control group, polysaccharide from Scutellaria barbata D. Don significantly increased the content of phospholipids, and decreased the content and ratio of cholesterol of erythrocyte membrane in S₁₈₀ tumor-bearing mice. Polysaccharide from Scutellaria barbata D. Don improved the rate of palmitic acid, oleic acid, linoleic acid and UFA/TFA in different degrees. CONCLUSION Through improving the phospholipids content, decreasing the content of cholesterol and the ratio of unsaturated fatty acid of erythrocyte membrane in S180 tumor-bearing mice, polysaccharide from Scutellaria barbata D. Don may improve the fluidity of the membrane, enhance the immune function of red blood cells, which exerts its antitumor effect.