HPLC测定三层共挤输液用袋中抗氧剂PEPQ的含量

Content Determination of Antioxidants PEPQ in Infusion of 3-layer Co-extrusion Bags by HPLC

关键词:  高效液相色谱法  三层共挤输液用袋  抗氧剂四(2,4-二叔丁基酚)-4,4-联苯基二亚磷酸酯

Key words:  HPLC  infusion of 3-layer co-extrusion bags  antioxidants PEPQ

摘要:

目的 建立三层共挤输液用袋中抗氧剂四(2,4-二叔丁基酚)-4,4-联苯基二亚磷酸酯(PEPQ)的含量测定法。方法 采用Dikma Diamonsil C₁₈色谱柱(250 mm×4.6 mm，5 μm)，流动相为乙腈-四氢呋喃(80∶20)，柱温30 ℃，检测波长270 nm。结果 抗氧剂PEPQ在0~200.0 μg·mL^-1内呈良好的线性关系(r=0.999)，抗氧剂PEPQ回收率为92.3%(RSD=1.97%)。结论 该方法准确、灵敏、简便，适用于测定三层共挤输液用袋中抗氧剂PEPQ的含量。

Abstract:

OBJECTIVE To develop a method for content determination of PEPQ infusion of 3-layer co-extrusion bags by HPLC. METHODS The analysis was performed on Dikma Diamonsil C₁₈(250 mm×4.6 mm, 5 μm) column, using acetonitrile-tetrahydrofuran(80∶20), Column temperature 30 ℃ and detection wavelength 270 nm. RESULTS The method has a linear range between 0-200.0 μg·mL^-1(r=0.999), The average recovery of antioxidants PEPQ was 92.3%(RSD=1.97%). CONCLUSION This method is accurate, sensitive, simple, it can be used for content determination of antioxidants PEPQ in infusion of 3-layer co-extrusion bags.