褪黑素对脑出血大鼠应激性胃黏膜损伤的保护作用

Protective Effect of Melatonin on Stress-induced Gastric Mucosa Injury in Rats of Cerebral Hemorrhage

关键词:  脑出血  应激  胃黏膜  SOD  MDA

Key words:  cerebral hemorrhage  stress  gastric mucosa  SOD  MDA

摘要:

目的 探讨褪黑素对脑出血大鼠应激性胃黏膜损伤的保护作用。方法 将60只健康成年SD大鼠随机分为空白对照组、模型组、奥美拉唑组、褪黑素组。空白对照组、模型组大鼠给予生理盐水灌胃，奥美拉唑组以奥美拉唑悬液灌胃，褪黑素组给予褪黑素灌胃，预防性给药，连续灌胃7 d，末次给药后禁食水24 h，除了空白对照组外，其余3组均制作脑出血大鼠模型，通过酶联免疫吸附法检测大鼠血清中超氧化物歧化酶(superoxidedismutase，SOD)、丙二醛(malonaldehyde，MDA)含量，大体观察大鼠胃黏膜病变并计算溃疡指数(UI)，HE染色光镜下观察胃黏膜组织形态学改变，并比较各组胃黏膜损伤的发病率。结果 褪黑素的治疗增加了SOD含量，降低MDA含量、降低大鼠胃黏膜UI及胃黏膜损伤的发病率(P<0.05)。结论 褪黑素可通过清除氧自由基而对应激性胃黏膜起到保护作用。

Abstract:

OBJECTIVE To investigate the protective effect of melatonin on irritability gastric mucosa injury in rats of cerebral hemorrhage. METHODS All 60 adult healthy male Sprague-Dawley rats were randomly divided into blank control group, model group, omeprazole group, melatonin group. The rats received respective treatments by prophylactic administration for 7 d: the blank control group and model group received normal saline by gavage; the omeprazole group received water suspension of omeprazole by gavage; melatonin group received melatonin by gavage. After the last administration, followed by 24 h of fasting(without water deprivation), a rat model of cerebral hemorrhage was established in all groups except blank control, After the model was established, serum levels of MDA, SOD were determined by enzyme-linked immunosorbent assay, gastric morphology was observed; gastric ulcer index (UI) was calculated; and the gastric mucosal tissue HE staining microscopy results and the incidence of gastric mucosal injury were compared. RESULTS Melatonin increased the SOD content and reduced MDA content, which reduced the rat gastric mucosa UI and the incidence of gastric mucosal injury (P<0.05). CONCLUSION Melatonin may protect stress gastric mucosal through clear oxygen free radicals.