响应面法优化超声波提取昆仑雪菊总黄酮的工艺研究

Optimization of Ultrasonic Extraction of Total Flavonoids from Coreopsis Tinctoria Nutt. Using Response Surface Methodology

关键词:  昆仑雪菊  总黄酮  超声波提取  单因素试验  Box-Behnken响应面

Key words:  Coreopsis tinctoria Nutt.  total flavonoids  ultrasonic extraction  single fator test  Box-Behnken response surface methodology

摘要:

目的 优化昆仑雪菊的超声提取工艺。方法 以总黄酮提取量为指标，在单因素试验基础上，通过Box-Behnken响应面法考察乙醇体积分数、料液比、超声时间和温度对总黄酮提取效果的影响。结果 最佳超声提取工艺为乙醇体积分数65%，料液比40 g·mL^-1，超声时间34 min，超声温度62 ℃；总黄酮提取量为207.32 mg·g^-1(RSD=1.45%)，与预测值(208.81 mg·g^-1)的偏差较小。结论 优选的提取工艺可行，为昆仑雪菊的开发应用提供实验依据。

Abstract:

OBJECTIVE To optimize ultrasound extraction technology of total flavonoids from Coreopsis tinctoria Nutt.. METHODS With the content of total flavonoids as index, effects of ethanol concentration, solid-liquid ratio, ultrasonic time and temperature on extraction technology were investigated by single factor tests and Box-Behnken response surface methodology. RESULTS Optimum ultrasound extraction technology was as following: ultrasonic extracted 34 min with 40 times of 65% ethanol to the herb at 62 ℃. Under these conditions, the average extraction rate of flavonoids was 207.32 mg·g^-1(RSD=1.45%), which was very close to the predicted value of 208.81 mg·g^-1. CONCLUSION This optimized technology is stable and feasible to provide a basis for development and application of total flavonoids in Coreopsis tinctoria Nutt..