HPLC测定银花芒果片中绿原酸、芒果苷和橙皮苷的含量

Determination of Chlorogenic Acid, Mangiferin and Hesperidin in Yinhua Mangguo Tablets by HPLC

关键词:  银花芒果片  绿原酸  芒果苷  橙皮苷  高效液相色谱法

Key words:  Yinhua Mangguo tablets  chlorogenic acid  mangiferin  hesperidin  HPLC

摘要:

目的 建立HPLC同时测定银花芒果片中绿原酸、芒果苷和橙皮苷的含量。方法 采用Waters SunFire^TM C₁₈ (4.6 mm×150 mm，5 μm)色谱柱；流动相为甲醇(A)-0.2%磷酸水溶液(B)，梯度洗脱，流速为1.0 mL·min^-1，检测波长为327 nm(0~10 min，绿原酸)，258 nm(10~20 min，芒果苷)，283 nm(20~40 min，橙皮苷)。结果 绿原酸进样量在0.140~2.516 μg内线性关系良好(r=0.999 9，n=6)，平均回收率为99.4%，RSD为1.2%(n=9)；芒果苷进样量在0.042~0.761 μg内线性关系良好(r=0.999 9，n=6)，平均回收率为99.9%，RSD为1.4%(n=9)。橙皮苷进样量在0.076~1.361 μg内线性关系良好(r=0.999?8，n=6)，平均回收率为99.9%，RSD为0.7%(n=9)。结论 该方法简便、快捷、准确，可用于该制剂的质量控制。

Abstract:

OBJECTIVE To establish an HPLC method for determination of chlorogenic acid, mangiferin and hesperidin in Yinhua Mangguo tablets. METHODS Waters SunFire^TM C₁₈(4.6 mm×150 mm, 5 μm) column was used. The mobile phase was methanol-water containing 0.2% phosphoric acid with gradient elution mode at the flow rate of 1.0 mL·min^-1; the detection wavelength was 327 nm(0-10 min) for chlorogenic acid, 258 nm(10-20 min) for mangiferin, 283 nm(20-40 min) for hesperidin. RESULTS Chlorogenic acid had a good linearity in the range of 0.140-2.516 μg, the average recovery was 99.4% with RSD of 1.2% (n=9). mangiferin had a good linearity in the range of 0.042-0.761 μg, the average recovery was 99.9% with RSD of 1.4% (n=9). hesperidin had a good linearity in the range of 0.076-1.361 μg, the average recovery was 99.9% with RSD of 0.7%(n=9). CONCLUSION The method is simple, accurate and reproducible. It can be used to determine chlorogenic acid, mangiferin and hesperidin in Yinhua Mangguo tablets.