仙茅、淫羊藿配伍对肾阳虚小鼠血浆ACTH和cAMP含量的影响

Effect of Compatibility of Curculigo and Epimedium to ACTH and cAMP on the Kidney Yang Deficiency Mice

关键词:  二仙药对  配伍  补肾壮阳

Key words:  Erxian drug couple  compatibility  invigorate the kidney and strengthen yang

摘要:

目的 比较仙茅、淫羊藿若干配伍方对肾阳虚小鼠补肾壮阳作用的疗效，并初探其作用机制。方法 在总量恒定的情况下，以仙茅与淫羊藿的经典比例1∶1为基准，向两侧扩展，得到若干水提及醇提的配伍方，小鼠灌胃给予腺嘌呤复制肾阳虚模型，同时以5 g·kg^-1剂量灌胃给二仙汤各配伍方，给药3周后，以小鼠体质量、自主活动次数、脏器指数、血浆垂体促肾上腺皮质激素(adrenocorticotropic hormone，ACTH)、环磷酸腺苷(cyclic adenosine monophosphate，cAMP)含量为指标，比较各配伍方的疗效。结果 与模型组相比，给药3周后，水提1∶2组、醇提仙茅组、醇提1∶1组、醇提1∶2组体质量增加非常显著(P<0.01)；水提1∶2组、水提淫羊藿组、醇提1∶2小鼠自主活动次数均显著增加(P<0.01)；水提淫羊藿组、水提1∶2组、醇提1∶2组脏器指数增大较为明显；水提2∶1组、水提1∶2组、醇提1∶2组小鼠血浆中cAMP活力显著升高(P<0.01)，ACTH含量明显增加(P<0.05或P<0.01)。结论 二仙汤各配伍方中，水提1∶2组、醇提1∶2组补肾壮阳的作用最为显著。

Abstract:

OBJECTIVE To obsearve the effect of curculigo and epimedium some compatibility of kidney yang deficiency mice role and Preliminary its mechanism of action. METHODS Under the total dosage being constant, several compatible prescriptions were obtained by uniform design with Curculigo and Epimedium classical proportion 1∶1 as a reference. Their extracts were obtained being by water and alcohol. Mice fed with adenine copy kidney Yang model, while 5 g·kg^-1 dose intragastric administration to each compatibility prescripti-ons, after three weeks administration to mice, body weight, locomotor activity, organ index, plasma pitu-itary adrenocorticotropic hormone (ACTH), cyclic adenosine monophosphate (cAMP) content were taken as indexes, comparing the efficacy of different compatible prescriptions. RESULTS Compared with the model group, after three weeks experiment, in water extract 1∶2 group, water extraction Curculigo group, alcohol extract of 1∶2 and 1∶1 groups, body weight of mice increased significantly(P<0.01); independent activity of water extract 1∶2 groups, water extraction Epimedium groups, alcohol extract 1∶2 was increased significantly in the number of mice(P<0.01); water extraction Epimedium group, alcohol extract 1∶2 and water extract 1∶2 group, viscera index increased more obviously; alcohol extract 1∶2 groups, water extract 2∶1 and 1∶2 group, plasma cAMP vitality of mice were increased significantly(P<0.01), and ACTH were increased significantly(P<0.05 or P<0.01). CONCLUSION The different compatibility prescriptions of Erxian soup, water extract 1∶2 group and alcohol extract 1∶2 group the effect of invigorate the kidney and strengthen yang is most significant.