野菊花提取物对糖尿病肾病大鼠的影响及作用机制

Effects and Mechanism of Extracts of Chrysanthemi Indici Flos on Rats with Diabetic Nephropathy

关键词:  野菊花提取物  糖尿病肾病  醛糖还原酶

Key words:  extracts of Chrysanthemi Indici Flos  diabetic nephropathy  aldose reductase

摘要:

目的 观察野菊花提取物对糖尿病肾病大鼠的影响及作用机制。方法 将48只SD大鼠，♀，随机分为正常对照组、模型对照组和野菊花低、中、高剂量组和阳性药依帕司他组(n=8)。除正常对照组外，其余各组大鼠腹腔注射链脲佐菌素35 mg·kg^-1，成功造模后野菊花组分别给予1，2，4 g·kg^-1野菊花提取物，依帕司他组给予10 mg·kg^-1依帕司他，连续灌胃8周后，检测大鼠血糖、体质量、肾脏脏体比、24 h尿白蛋白量、肾组织超氧化歧化酶(SOD)、过氧化氢酶(CAT)的活性及丙二醛(MDA)的含量；HE染色检测肾脏病理改变，ELISA法检测醛糖还原酶(AR)活性并测定其基因表达。结果 与正常对照组相比，模型对照组上述指标除CAT外均有显著改变；与模型对照组相比，野菊花能明显降低大鼠血糖水平、AR活性及其基因表达，明显减少24 h尿白蛋白，升高肾组织SOD、CAT活性，显著降低MDA含量。结论 野菊花提取物对大鼠糖尿病肾病有一定治疗作用，在调节血糖的同时，可能通过提高机体抗氧化能力、减少肾脏AR活性与基因表达以抑制多元醇通路的激活发挥其肾功能的保护作用。

Abstract:

OBJECTIVE To explore the effects and mechanism of extracts of Chrysanthemi Indici Flos on rats with diabetic nephropathy. METHODS Forty eight SD rats, ♀, were randomly divided into normal control group, model control group, epalrestat group and three doses of Chrysanthemi Indici Flos groups(n=8). Diabetes model was established by streptozotocin(35 mg·kg^-1, i.p). Extracts of Chrysanthemi Indici Flos(1, 2, 4 g·kg^-1, respectively) were oral administrated for 8 weeks. The 10 mg·kg^-1 epalrestat was administrated in epalrestat group. The blood glucose, weight and the contents of superoxide dismutase(SOD), catalase(CAT) and malonaldehyde(MDA) of kidney were measured. The pathological changes in the kidney were measured by hematoxylin and eosin stain. The activity of aldose reductase(AR) in kidneys was detected by ELISA and the change of AR gene expression was also determined. RESULTS Compared with normal control group, the indexes mersured above except the activity of CAT in model control group changed significantly. Compared with model control group, extracts of Chrysanthemi Indici Flos significantly reduced the level of blood glucose and the content of urine protein and MDA in kidney. They also increased the activity of SOD and the content of MDA in kidney. The activity and gene expression of AR were both obviously decreased. CONCLUSION Chrysanthemi Indici Flos could ameliorate renal dysfunction in rats with diabetic nephropathy by controlling the blood glucose, reducing the oxidative stress, and inhibiting the activation of polyol-pathway.