通心络对局灶性脑缺血再灌注损伤大鼠的保护作用

Protective Effect of Tongxinluo Capsule on Focal Cerebral Ischemia-reperfusion Injury in Rats

关键词:  通心络  脑缺血再灌注损伤  Fas蛋白

Key words:  Tongxinluo  cerebral ischemia-reperfusion injury  Fas protein

摘要:

目的 探讨通心络对局灶性脑缺血再灌注损伤大鼠的保护作用。方法 将60只SD大鼠随机分成假手术组、模型组、通心络低、中、高剂量组(0.3，0.6，1.2 g·kg^-1)，每组12只。线栓法制作大鼠脑缺血再灌注模型，于缺血30 min后再灌注，24 h后进行神经行为学评分，采用TTC法测量脑梗死体积，HE染色观察脑组织的病理改变，免疫组化法观察Fas蛋白的表达。结果 与模型组比较，通心络低、中、高剂量组均能显著改善大鼠的神经系统损伤症状，缩小脑梗死灶，明显下调Fas蛋白的表达(P<0.01)；并且通心络高剂量组与低剂量组比较，差异具有统计学意义(P<0.05)。结论 通心络可通过抑制Fas蛋白的表达，减轻脑缺血后神经功能损伤，从而保护脑组织。

Abstract:

OBJECTIVE To investigate the protective effect of Tongxinluo capsule on focal cerebral ischemia-reperfusion injury in rats. METHODS Sixty SD rats were randomly divided into the sham-operative group, ischemia-reperfusion group, Tongxinluo capsule low-dose group(0.3 g·kg^-1), medium-dose group(0.6 g·kg^-1) and high-dose group(1.2 g·kg^-1), respectively (n=12). The ischemia reperfusion model rats were induced using suture-occluded method, 30 min of ischemia-reperfusion. after cerebral ischemia 30 min and reperfusion 24 h, to evaluate the score of neurological behavior, using the TTC method measuring the cerebral infarction volume, HE staining to observe the pathological changes of brain tissue and immunohistochemical method to observe the expression of Fas protein. Results Compared with ischemia-reperfusion group, Tongxinluo low, medium and high dose group could significantly improve the symptoms of nervous system damage in rats, reduce cerebral infarcts, Fas protein expression were obviously down-regulated(P<0.01). And compared with Tongxinluo low-dose group, all of them were significantly different in Tongxinluo high-dose group(P<0.05). Conclusion Tongxinluo capsule can inhibit the expression of Fas protein, alleviate nerve function damage after cerebral ischemia, so as to protect the brain tissue.