益母草流浸膏的指纹图谱和多指标成分含量测定方法研究

Fingerprint Method and Multi-Component Quantification Analysis Method Study of Leonurus Liquid Extract

关键词:  益母草流浸膏  指纹图谱  含量测定

Key words:  Leonurus liquid extract, fingerprint, content determination

摘要:

目的 建立益母草流浸膏的指纹图谱方法及多指标成分含量测定方法。方法 通过水饱和正丁醇萃取，采用HPLC，Kromasil C₁₈(250 mm×4.6 mm，5 μm)色谱柱，柱温30 ℃，流动相为乙腈-0.1%甲酸，梯度洗脱，流速为1.0 mL·min^-1，检测波长为275 nm。结果 建立益母草流浸膏的HPLC指纹图谱，得到共有峰6个，指认其中3个并测定含量，对羟基苯甲酸、丁香酸和盐酸益母草碱分别在进样量3.952~790.4 ng(r=1.000 0)、10.427~2 085.4 ng(r=1.000 0)和6.983~1 396.6 ng(r=0.999 9)内呈良好的线性关系；平均回收率分别为98.6%、97.8%和97.9%，RSD分别为1.5%、2.0%和1.8%。结论 该指纹图谱特征性明显，方法简便，重复性好，可为益母草流浸膏提供有效的鉴别依据；多指标含量测定方法可同时控制指纹图谱中3个成分的含量，方法简便、准确，可为综合评价和控制产品质量提供依据。

Abstract:

OBJECTIVE To establish the fingerprint chromatogramand multi-component quantification analysis method of Leonurus liquid extract. METHODS Extracted by n-Buthyl alcohol, the extract was analyzed on Kromasil C₁₈ column (250 mm×4.6 mm, 5 μm)and the temperature of column was 30 ℃. The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution. The flow rate was 1.0 mL·min^-1. The detection wavelength was at 275 nm. RESULTS HPLC fingerprint of characteristic components of Leonurus liquid extract was established for the first time, 6 common peaks were figured out, and 3 of them were identified and determinated. P-hydroxybenzoic acid, syringic acid, and hydrochloric acid leonurine showed good linearities in the ranges of 3.952-790.4 ng(r=1.000 0), 10.427-2 085.4ng(r=1.000 0) and 6.983-1 396.6 ng(r=0.999 9), the average recoveries were 98.6%, 97.8% and 97.9%, with RSD of 1.5%, 2.0% and 1.8%, respectively. CONCLUSION The HPLC characteristic fingerprint for the identification of Leonurus liquid extract was specific, simple and reproducible. The established multi-component quantification analysis method could be used to control the quality of Leonurus liquid extract, and provided the basis of comprehensive evaluation and quality control.