HPLC同时测定五福化毒丸中靛蓝、靛玉红和甘草酸的含量

Simultaneous Determination of Indigo, Indirubin and Glycyrrhizic Acid in Wufu Huadu Pill by HPLC

关键词:  五福化毒丸  靛蓝  靛玉红  甘草酸  高效液相色谱法

Key words:  Wufu Huadu pill  indigo  indirubin  glycyrrhizic acid  HPLC

摘要:

目的 建立同时测定五福化毒丸中靛蓝、靛玉红和甘草酸含量的高效液相色谱法。方法 采用Eclipse XDB-C₁₈(250 mm×4.6 mm，5 μm)色谱柱，流动相为甲醇-0.2 mol·L^-1乙酸铵-冰醋酸(65∶35∶1)，流速为1.0 mL·min^-1，检测波长为288，250 nm，柱温：25 ℃。结果 靛蓝、靛玉红和甘草酸依次在2.501~25.01，0.633~6.330，5.10~51.02 μg·mL^-1内与峰面积呈良好线性关系，相关系数r分别为1.000 0，0.999 5，1.000 0，加样回收率(n=9)分别为98.3%，98.4%和98.2%。结论 本方法简便，准确，重复性好，可用于该制剂的质量控制。

Abstract:

OBJECTIVE To develop an HPLC method for simultaneous determination of indigo, indirubin and glycyrrhizic acid in Wufu Huadu pill. METHODS An Eclipse XDB-C₁₈ column(250 mm×4.6 mm, 5 μm) was adopted. The mobile phase was methanol-0.2 mol·L^-1 ammonium acetate-glacial acetic acid(65∶35∶1) at the flow rate of 1.0 mL·min^-1. The UV-detection wavelength was at 250, 288 nm with the column temperature of 25 ℃. RESULTS Calibration curves were found to be linear in the ranges of 2.501-25.01 μg·mL^-1 for indigo, 0.633-6.330 μg·mL^-1 for indirubin and 5.10-51.02 μg·mL^-1 for glycyrrhizic acid. The correlation coefficients were 1.000 0, 0.999 5, 1.000 0. The average recoveries(n=9) of indigo, indirubin and glycyrrhizic acid were 98.3%, 98.4% and 98.2%. CONCLUSION The method is simple, accurate and reproducible. It could be reliable for the quality control of Wufu Huadu Pill.