芦丁对脂多糖诱导急性肺损伤小鼠的抗氧化应激作用

Effect of Rutin on Oxidative Stress of Acute Lung Injury Induced by Lipopolysaccharide

关键词:  芦丁  脂多糖  急性肺损伤  氧化应激

Key words:  rutin  lipopolysaccharide(LPS)  acute lung injury  oxidative stress

摘要:

目的 探讨芦丁对脂多糖(lipopolysaccharide，LPS)诱导的小鼠急性肺损伤氧化应激失衡的作用。方法 将50只C57小鼠随机分为5组(n=10)，分别为对照组、模型组和芦丁低、中、高剂量(25，50，100 mol·kg^-1)组。LPS注射6 h后测小鼠血气，取肺组织进行病理观察，测肺组织的湿干比、丙二醛(MDA)含量、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)活性。结果 与对照组相比，模型组的肺组织病理改变和肺组织的湿干比，二氧化碳分压[p(CO₂)]，呼吸频率(RR)和MDA含量明显增加，而氧分压[p(O₂)]和pH值、CAT、GPx和SOD活性明显降低。与模型组相比，芦丁组的肺组织病理改变和肺组织湿干比，p(CO₂)、呼吸率和MDA含量呈浓度依赖性减少，而p(O₂)和pH值、CAT、GPx和SOD活性呈浓度依赖性增高。结论 芦丁可呈浓度依赖性抑制氧化应激而减轻脂多糖诱导的小鼠急性肺损伤。

Abstract:

OBJECTIVE To investigate the effects of rutin on oxidative stress of acute lung injury induced by lipopolysaccharide(LPS). METHODS Fifty male C57 mice were randomly divided into five groups: control group, model group, and rutin low, middle, high(25, 50, 100 mol·kg^-1) groups. After 6 h of LPS injected, collecting arterial blood for blood gas analysis and lung tissue to examine pathological change, Wet/Dry, the expression of MDA and the activity of CAT, GPx and SOD. RESULTS Compared with the control group, the pathological change and Wet/Dry of lung tissue, the p(CO₂) and respiratory rate(RR), the expression of MDA in lung tissue were dose-increased, while the p(O₂), pH and the activity of CAT, GPx and SOD were decreased. compared with the model group, the pathological change and Wet/Dry of lung tissue, the p(CO₂) and RR, the expression of MDA in lung tissue in rutin groups were concentration-dependent decreased, while the p(CO₂), pH and the activity of CAT, GPx and SOD were concentration-dependent increased. CONCLUSION Rutin pretreatment can concentration- dependent decrease LPS induced acute lung injury by suppressing oxidative stress.