芹菜素对L02细胞非酒精性脂肪性肝炎模型炎症反应及胰岛素抵抗的改善作用

Effect of Apigenin on Inflammatory Response and Insulin Resistance of L02 Cell Nonalcoholic Steatohepatitis Model

关键词:  芹菜素  L02细胞  非酒精性脂肪性肝炎  炎症反应  胰岛素抵抗

Key words:  apigenin  L02 cell  nonalcoholic steatohepatitis  inflammatory response  insulin resistance

摘要:

目的 探讨芹菜素对游离脂肪酸(free fatty acids，FFA)诱导的L02细胞的非酒精性脂肪性肝炎(nonalcoholic steatohepatitis，NASH)模型的炎症反应和胰岛素抵抗的治疗活性。方法 FFA处理L02细胞24 h，建立NASH细胞模型；MTT法检测细胞生长抑制率并制定安全给药浓度；油红O染色法观察细胞脂质沉积情况；Elisa法检测细胞的白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)和葡萄糖转运蛋白-4(GLUT-4)含量；Western-blot法及Elisa法检测细胞的胰岛素受体(IR)的含量。结果 MTT试验显示芹菜素最高给药浓度(280.00 μg·mL^-1)下，细胞生长抑制率仅为18.8%；与模型组比较，芹菜素高、中、低剂量组(250，150，100 μg·mL^-1)GLUT-4和IR的含量相均明显提高(P<0.01)，而高、中剂量组的IL-8、TNF-α含量和脂变率均明显下降(P<0.01)。结论 芹菜素可调节细胞脂变率和IL-8、TNF-α、GLUT-4、IR的含量，改善FFA诱导的L02细胞NASH模型的胰岛素抵抗及炎症反应，且具有极小的细胞毒性。

Abstract:

OBJECTIVE To explore the effect of apigenin on inflammatory response and insulin resistance of L02 cell nonalcoholic steatohepatitis(NASH) model induced by free fatty acids(FFA). METHODS L02 cells were treated with FFA for 24 h to establish NASH model. The cell proliferation inhibition ratio was measured by MTT method and the safe drug concentration of apigenin was determineed. Oil-red staining was used to observe the fat deposition in cells. The content of interleukin-8(IL-8), tumor necrosis factor-α(TNF-α)and glucose transporter-4(GLUT-4) were detected by Elisa. The content of insulin receptor(IR) was detected by Western-blot and Elisa. RESULTS MTT test showed a cell proliferation inhibition ratio of 18.8% under the maximum concentration of apigenin(280.0 μg·mL^-1). Compared with modle group, the content of GLUT-4 and IR of high, middle and low dose group of apigenin(250，150，100 μg·mL^-1) increased obviously(P<0.01), but the content of IL-8 and TNF-α and fat deposition ratio of high and middle dose group decreased obviously(P<0.01). CONCLUSION Apigenin can improve the insulin resistant and inflammatory response of L02 cell NASH model by adjusting the fat deposition ratio and the contents of IL-8, TNF-α, GLUT-4 and IR，and it has tinny cytotoxicity.