红景天主要成分对小鼠免疫细胞的促增殖转化作用

Major Efficacy Component of Rhodiola Stimulate Proliferation and Transformation on Mouse Immune Cells

关键词:  红景天苷  苷元酪醇  络塞维  T/B细胞  白细胞  细胞转化

Key words:  salidroside  tyrosol  rosavin  T/B cells  white blood cells  transformation of cell

摘要:

目的 观察红景天中主要成分红景天苷、苷元酪醇、络塞维对小鼠免疫系统的保护作用。方法 以Giemsa染色法、MTT法及NO检测法等为研究手段，分析不同浓度红景天苷、酪醇、络塞维对小鼠外周血T、B淋巴细胞、小鼠外周血白细胞及小鼠腹腔巨噬细胞等具有代表意义的免疫细胞活性及功能的影响。结果 红景天苷能够促进T/B淋巴细胞的转化及增殖，对外周血白细胞及腹腔巨噬细胞的吞噬能力有显著的提升作用。苷元酪醇(62.5~250 μg·mL^-1)能够特异性的刺激B淋巴细胞的增殖和转化(P<0.05)，且作用效果高于阳性药物组。络塞维则对静止型T细胞向母细胞转化具有显著的促进作用(P<0.05)。结论 不同红景天主要成分在体外会对不同的免疫靶细胞起作用，具有细胞免疫和体液免疫功能，同时能提高单核细胞的吞噬能力，免疫活性广泛。

Abstract:

OBJECTIVE To observe the immunopotentiation of salidroside, tyrosol and rosavin of major ingredient of Rhodiola on mouse immune system. METHODS LPS-induced and ConA-induced mouse peripheral blood lymphocyte proliferation and transformation, and phagocytosis of Rhodiola on peripheral blood leukocyte and peritoneal macrophage were analysed by MTT assay, Giemsa staining and NO detection kit. RESULTS Compared with solvent control group, the salidroside group could increase proliferation and transformation of T/B cells significantly, and also improved phagocytosis of peripheral blood leukocyte and peritoneal macrophage(P<0.05). Moreover, there was significant difference between the tyrosol group (62.5-250 μg·mL^-1) and levamisole hydrochloride (positive drug) group on LPS-induced B cells. And rosavin could obviously increase the transformation of mouse T cells compared with solvent control group. CONCLUSION Three main active substance of Rhodiola can enhance mouse non-specific and specific immune response, and activity of T/B cells and macrophage.