苦参及苦参碱对小鼠肝脏和神经行为学的影响

Study of Hepatotoxicity and Neural Behavioral Changes of Sophora Flavescens and Matrine in Mice

关键词:  苦参  苦参碱  肝脏毒性  神经行为学

Key words:  Sophora flavescens  matrine  hepatotoxicity  neural behavior

摘要:

目的 观察苦参及苦参碱对小鼠肝脏毒性及神经行为学功能的影响。方法 50只ICR小鼠随机分为空白组、苦参1.5，15 g·kg^-1剂量组，苦参碱10，40 mg·kg^-1剂量组，每天给药1次，连续灌胃60 d；首次给药前和末次给药后测定小鼠自主活动及爬竿法平衡协调能力，末次给药后，测定小鼠体质量、血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(AKP)水平及肝组织SOD活性和MDA含量。结果 与空白组相比，给药组小鼠体质量降低、自主活动数减少，平衡协调能力下降；各给药组AST、ALT、AKP活性均有差异，其中苦参1.5 g·kg^-1，苦参碱10，40 mg·kg^-1组AST、ALT、AKP活性有显著性差异(P<0.05或P<0.01)；肝组织SOD活性无明显变化，MDA含量显著上升。结论 小鼠连续灌胃苦参及苦参碱可引起肝脏毒性和神经行为学的改变。

Abstract:

OBJECTIVE To investigate the effects of Sophora flavescens and the matrine on liver toxicity and neural behavioral changes in mice. METHODS Fifty ICR mice were randomly divided into control group, Sophora flavescens 1.5, 15 g·kg^-1 groups, matrine 10, 40 mg·kg^-1 groups. Mice in experiment were induced by intragastrical administration once a day for 60 days; the control group were given with the same volume of potable water. Neurobehavioral indexes which included balance, coordination ability and locomotor activity were determined before the first administration and after the last administration. The balance and coordination ability were performed by climbing-pole test. The activity changes of AST, ALT, AKP in plasma, the SOD activity and the MDA levels in liver were detected. RESULTS Compared with the control group, body weight, locomotor activity, balance and coordination ability in experimental groups exhibited a significant decrease. Meanwhile the activities of AST, ALT and AKP of Sophora flavescens 1.5 g·kg^-1 group, matrine 10 and 40 mg·kg^-1 groups increased with significantly statistical difference(P<0.05 or P<0.01). The contents of MDA decreased significantly while SOD activity had no change. CONCLUSION Sophora flavescens and matrine can cause hepatotoxicity and neural behavioral changes to mice with repeated administration.