| 引用本文: | 刘亚丽,魏韶锋,宋永贵,李伟,张凌,杨世林,王萌,苏丹.RRLC-ESI-MS/MS分析白头翁皂苷在大鼠体内的组织分布[J].中国现代应用药学,2016,33(1):6-11. |
| LIU Yali,WEI Shaofeng,SONG Yonggui,LI Wei,ZHANG Ling,YANG Shilin,WANG Meng,SU Dan.Distribution Analysis of Saponins in Pulsatilla Chinensis(Bunge) Regel in Rats’ Tissues by RRLC-ESI-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(1):6-11. |
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| 摘要: |
| 目的 研究白头翁皂苷在大鼠体内的分布规律和蓄积情况。方法 以300 mg·kg-1白头翁皂苷提取物均匀分散混悬液单次灌胃给予大鼠后，采用RRLC-ESI-MS/MS对白头翁皂苷中的有效成分B3，BD，B7，B10，B11在动物体内的组织分布情况进行考察。结果 白头翁皂苷在动物体内的分布迅速且广泛，给药0.25 h后，各组织脏器中即可检测到较高水平的药物浓度。5种皂苷在大部分组织器官中的达峰时间为30 min，给药2 h后，白头翁皂苷的浓度逐渐下降，给药6?h后，药物基本消除。在动物脑中亦可检测到药物的存在。结论 白头翁皂苷在体内不易蓄积，主要分布在心脏、肝、肺、肾、小肠等器官，心脏中药物浓度最高，且可以透过血脑屏障。 |
| 关键词: 白头翁皂苷 RRLC-ESI-MS/MS 组织分布 |
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| 基金项目:国家自然科学基金(81460594)；江西省自然科学基金(20151BAB215038)；江西省卫计委科研项目(2015A054)；江西中医药大学博士启动基金(2010BS2037、2014BS019) |
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| Distribution Analysis of Saponins in Pulsatilla Chinensis(Bunge) Regel in Rats’ Tissues by RRLC-ESI-MS/MS |
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LIU Yali1, WEI Shaofeng1, SONG Yonggui2, LI Wei2, ZHANG Ling2, YANG Shilin2, WANG Meng1, SU Dan2
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1.Science and Technology College, Jiangxi University of Chinese Traditional Medicine, Nanchang 330025, China;2.The National Pharmaceutical Engineering Center(NPEC) for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Chinese Traditional Medicine, Nanchang 330006, China
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| Abstract: |
| OBJECTIVE To research the distribution and accumulation of pulchinenosides(B3, BD, B7, B10, B11) in rats. METHODS The tissue distribution had been investigated after 300 mg·kg-1 oral administration of pulsatilla chinensis (bunge) regel saponins(PRS) evenly dispersed suspension by RRLC-ESI-MS/MS. RESULTS PRS exhibited rapid and extensive distribution, and high level concentrations could be detected in rats’ tissues after 0.25 h for oral administration. Tmax of 5 components in the tissues were about 30 min. The concentrations declined gradually after 2 h and a large proportion of PRS were almost eliminated after 6 h. PRS could also be detected in the brain. CONCLUSION PRS is difficult to accumulate in vivo. The medicine mainly distributes in heart, liver, lungs, spleen and small intestine, and it is highest in the heart than in other tissues, and it can transmitte across blood-brain barrier. |
| Key words: Pulsatilla chinensis (Bunge) regel saponins RRLC-ESI-MS/MS tissue distribution |
