裸花紫珠抑制NO生成活性及其活性部位的HPLC-DAD-ESI-MS研究

潘争红; 黄思思; 宁德生; 谷陟欣; 黄胜; 颜冬兰; 谢运昌; 李典鹏

引用本文:	潘争红,黄思思,宁德生,谷陟欣,黄胜,颜冬兰,谢运昌,李典鹏.裸花紫珠抑制NO生成活性及其活性部位的HPLC-DAD-ESI-MS研究[J].中国现代应用药学,2015,32(12):1463-1466.
	PAN Zhenghong,HUANG Sisi,NING Desheng,GU Zhixin,HUANG Sheng,YAN Donglan,XIE Yunchang,LI Dianpeng.Inhibition Activity on Nitric Oxide Production of Callicarpa Nudiflora and HPLC-DAD-ESI-MS Analysis on Its Effective Part[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(12):1463-1466.

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裸花紫珠抑制NO生成活性及其活性部位的HPLC-DAD-ESI-MS研究
潘争红^1,2, 黄思思¹, 宁德生¹, 谷陟欣², 黄胜^2,3, 颜冬兰², 谢运昌¹, 李典鹏¹
1.广西壮族自治区?中国科学院，广西植物研究所，广西植物功能物质研究与利用重点实验室，广西桂林 541006;2.九芝堂股份有限公司，长沙 410008;3.海南九芝堂药业有限公司，海口 570311

摘要:

目的确定裸花紫珠抑制一氧化氮(NO)生成的活性部位，并建立一套基于液质联用(HPLC-DAD-ESI-MS)技术快速鉴定裸花紫珠有效部位中主要成分的方法。方法以脂多糖(LPS)诱导巨噬细胞RAW264.7生成一氧化氮(NO)作为筛选模型，采用液质联用(HPLC-DAD-ESI-MS)进行成分测定。结果裸花紫珠中低极性的乙酸乙酯部位和石油醚部位显示出较强的抑制NO活性，可能是其抗炎活性部位，黄酮及黄酮苷类是乙酸乙酯部位的主要成分，黄酮和二萜成分是石油醚部位的主要成分。结论研究结果为裸花紫珠的产品升级及其质量控制提供了参考依据。

关键词: 裸花紫珠一氧化氮抗炎 HPLC-DAD-ESI-MS

DOI：

分类号:

基金项目:广西科学研究与技术开发计划项目(桂科攻11107010-3-10)

Inhibition Activity on Nitric Oxide Production of Callicarpa Nudiflora and HPLC-DAD-ESI-MS Analysis on Its Effective Part

PAN Zhenghong^1,2, HUANG Sisi¹, NING Desheng¹, GU Zhixin², HUANG Sheng^2,3, YAN Donglan², XIE Yunchang¹, LI Dianpeng¹

1.Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China;2.Jiuzhitang Co., Ltd., Changsha 410008, China;3.Hainan Jiuzhitang Pharmaceutical Co., Ltd., Haikou 570311, China

Abstract:

OBJECTIVE To determine the active fractions of Callicarpa nudiflora on inhibitory of nitric oxide production, and to establish a method for identification of the main compositions of Callicarpa nudiflora by HPLC-DAD-ESI-MS technology. METHODS The inhibitory activity of nitric oxide production was carried out using lipopolysaccharide (LPS) to induce macrophage RAW264.7. The main compositions were determined by HPLC-DAD-ESI-MS technology. RESULTS The petroleum ether fraction and ethyl acetate fraction of Callicarpa nudiflora could inhibit the production of nitric oxide in lipopolysaccharide induced RAW264.7 cells (IC₅₀<25 μg·mL^-1). Flavonoids and flavonoid glycosides were the main constituents of ethyl acetate fraction, and the main constituents of petroleum ether fraction were flavonoids and diterpenoids. CONCLUSION The research provides a reference for the upgrading of products and the quality control of Callicarpa nudiflora.

Key words: Callicarpa nudiflora nitric oxide anti-inflammatory HPLC-DAD-ESI-MS