黄连胆汁炙前后对热证模型大鼠血清甲状腺激素水平和体温的影响

Influence on Serum Thyroid Hormone and Body Temperature Before and after Coptidis Rhizoma Processed with the Pig’s Bile on the Febrile Rats

关键词:  热证大鼠  黄连  胆黄连  甲状腺激素  体温

Key words:  febrile rats  Coptidis Rhizoma  the bile processed Coptidis Rhizoma  thyroid hormone  body temperature

摘要:

目的 观察黄连经猪胆汁炮制前后对热证模型大鼠血清中甲状腺激素水平及体温变化的影响。方法 采用口服附子、干姜、肉桂水煎液与皮下注射外源性致热因子(干酵母混悬液)，复制热证模型大鼠。灌胃给予黄连、猪胆汁和胆黄连药液，测定大鼠血清中游离三碘甲腺原氨酸(FT3)、血清游离甲状腺素(FT4)、逆-三碘甲腺原氨酸(rT3)及大鼠体温。结果 与空白对照组比较，模型组大鼠体温显著性增高(P<0.001)，血清FT3、FT4、rT3含量均显著性降低(P<0.01)；与模型组大鼠比较，3 h的猪胆汁组和胆黄连组体温显著性降低(P<0.01)，黄连、胆黄连可显著升高FT3含量(P<0.05)，猪胆汁可显著性降低FT4含量(P<0.05)，rT3无显著性变化。结论 猪胆汁和胆黄连在3 h内可拮抗大鼠体温的升高，黄连和胆黄连可拮抗热证大鼠血清中FT3含量的降低。

Abstract:

OBJECTIVE To study the effect of serum thyroid hormone and body temperature before and after Coptidis Rhizoma processed with the pig’s bile on the febrile rats. METHODS A febrile type model was established with Aconitum carmichaeli Debx., Zingiber officinale Rosc. and Cinnamomum cassia Presl. decoction in rats, and then were given an injection of dry yeast subcutaneously to produce and release pyrogen, and the body temperature of adjusting point was raised up, so the febrile model was established successfully. The febrile model rats were administrated with Coptidis Rhizoma, pig’s bile and the bile processed Coptidis Rhizoma, respectively. Recording the body temperature of every rat, blood sample were drawn for detection of serum FT3, FT4 and rT3. RESULTS Compared with the normal group, the model group had significantly higher levels of body temperature(P<0.001), but lower levels of serum FT3, FT4 and rT3(P<0.01). Compared with the model group, the body temperature after 3 h decreased significantly(P<0.01) in the pig’s bile group and the bile processed Coptidis Rhizoma group. Coptidis Rhizoma and the bile processed Coptidis Rhizoma could significantly increase the level of FT3(P<0.05), the pig’s bile could significantly reduce the level of FT4(P<0.05), there was no significantly difference on rT3. CONCLUSION The pig’s bile and the bile processed Coptidis Rhizoma can antagonize the increase of the body temperature of rats. Coptidis Rhizoma and the bile processed Coptidis Rhizoma can improve metabolism in febrile rats by antagonizing the decrease of FT3 levels.