藏药刺柏提取物对CCl4致大鼠肝纤维化的保护作用

Protective Effect of Juniperus Extract on CCl4-induced Liver Fibrosis of Rats

关键词:  刺柏提取物  肝纤维化  氧化应激  MMP-2

Key words:  Juniperus extract  liver fibrosis  oxidative stress  MMP-2

摘要:

目的 研究藏药刺柏提取物(juniperus extract，JE)对大鼠肝纤维化的保护作用和相关机制。方法 利用CCl₄建立大鼠肝纤维化模型，JE(25，50 mg·kg^-1)连续灌胃给药6周。给药结束后检测血清谷丙转氨酶(ALT)、天冬氨酸氨基转移酶(AST)以及肝组织超氧化物歧化酶(SOD)、丙二醛(MDA)含量；HE染色观察肝组织的变化；应用免疫组化法检测MMP-2的表达。结果 与模型组比较，JE组大鼠血清中ALT、AST水平降低(P<0.05)，同时肝组织中SOD含量增加(P<0.05或P<0.01)，50 mg·kg^-1 JE组MDA含量降低(P<0.05)；病理学研究表明，JE组大鼠肝纤维化程度明显改善，50 mg·kg^-1 JE组能明显抑制肝组织中MMP-2的表达(P<0.05)。结论 JE可能通过降低MMP-2的表达减少氧化应激，从而改善CCl4引起的肝纤维化损伤。

Abstract:

OBJECTIVE to explore the effects and possible mechanism of Juniperus extract(JE) on liver fibrosis. METHODS The liver fibrosis model was induced by CCl4, and JE were administered by gastric perfusion at 25, 50 mg·kg^-1 qd for 6 weeks, at the end of the experiment, the contents of alanine transaminase(ALT), aspartate aminotransferase(AST), superoxide dismutase(SOD), maleic dialdehyde(MDA) and the contents of PINP were measured in liver tissue, the expression of MMP-2 was observed by immunohistochemisty. The pathological changes of liver tissue were examined by HE. RESULTS JE(25, 50 mg·kg^-1) improved the liver function significantly through reducing the level of ALT, AST (P<0.05), and increasing the content of SOD (P<0.05 or 0.01), 50 mg·kg-1 JE significantly reduced the centent of MDA and the expression of MMP-2(P<0.05). CONCLUSION JE may inhibit the liver fibrosis induced by CCl4 by decreasing the oxidative stress, the mechanism possibly involves the inhibition of the expression of MMP-2.