| 引用本文: | 王丹仙,马月,方杰,胡毅翔,俞文英,余陈欢,应华忠.玉麦microRNAs类活性物质的筛选及功能分析[J].中国现代应用药学,2016,33(1):12-15. |
| WANG Danxian,MA Yue,FANG Jie,HU Yixiang,YU Wenying,YU Chenhuan,YING Huazhong.Identification of Bioactivity MicroRNAs Derived from Zea Mays and Their Regulating Target Function[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(1):12-15. |
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| 摘要: |
| 目的 鉴定玉麦中可吸收入血的microRNAs(miRNAs)类活性物质，并分析其靶基因功能。方法 采用二代高通量测序法，以正常小鼠血浆miRNAs为空白对照，测定喂食玉麦匀浆液小鼠血浆中miRNAs的总量，并与玉麦miRNAs比对，鉴定吸收入体内的玉麦miRNAs。采用TargetScan与MiRanda软件，预测玉麦miRNAs可能作用于人类mRNA靶基因，并通过Gene Ontology(GO)与Kyoto Encyclopedia of Genes and Genomes(KEGG)分析靶基因的相关功能和信号途径。结果 共鉴定出12个可吸收入体内的玉麦miRNAs。GO与KEGG分析显示这些miRNAs可能调控的靶基因及其参与的生物学功能包括胰岛素信号通路、癌症通路、MAPK通路等相关信号通路，其中miR4995、miR156a、miR396e为玉麦可能的主要核酸活性物质。结论 miRNAs可能为玉麦中的一种新的活性物质。 |
| 关键词: 药效物质 高通量测序 靶基因 |
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| 基金项目:国家自然科学基金项目(81473339)；浙江省卫生高层次人才培养项目 |
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| Identification of Bioactivity MicroRNAs Derived from Zea Mays and Their Regulating Target Function |
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WANG Danxian1,2, MA Yue2, FANG Jie1,2, HU Yixiang2, YU Wenying2, YU Chenhuan2, YING Huazhong2
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1.Pharmacy College, Zhejiang Chinese Medical University, Hangzhou 310053, China;2.Zhejiang Academy of Medical Sciences, Zhejiang Key Laboratory of Experimental Animal and Safety Evaluation, Hangzhou 310013, China
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| Abstract: |
| OBJECTIVE To investigate microRNAs(miRNAs) derived from Zea mays and their target gene function. METHODS MiRNA profiles of Zea mays, in plasma of normal mice and Zea mays extract-treated mice were detected by using next generation sequencing, and then the miRNAs which were took into the organism were validated with miRNA database of Zea mays. Human mRNA database as targets was used to predicte target genes of miRNAs by TargetScan and MiRanda analysis, while the target gene function was considered by Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis. RESULTS Twelve miRNAs were identified. For the KEGG pathway, their target gene mainly involved in insulin signaling pathway, MAPK pathway and peritumoral pathways by GO and KEGG analysis. The results of miRNAs-gene-network showed that miR4995, miR156a, miR396e might be the main pharmacodynamic substance. CONCLUSION MiRNAs might act as a bioactivity substance in the Zea mays. |
| Key words: pharmacodynamic material high-through put sequencing target gene |
