枳壳及其制剂血府逐瘀胶囊中柚皮苷和新橙皮苷的含量测定

Determination of Naringin and Neohesperidin in Aurantii Fructus and its Preparation Xuefu Zhuyu Capsules

关键词:  枳壳  血府逐瘀胶囊  柚皮苷  新橙皮苷  高效液相色谱法

Key words:  Aurantii Fructus  Xuefu Zhuyu Capsules  naringin  neohesperidin  HPLC

摘要:

目的 建立HPLC同时测定枳壳和血府逐瘀胶囊中柚皮苷和新橙皮苷的含量。方法 色谱柱为SunFire^TM C₁₈(250 mm×4.6 mm，5 μm)；流动相为乙腈-水(20∶80)，磷酸调pH至2.5；流速为1.0 mL·min^-1；检测波长为283 nm。结果 柚皮苷在0.166~2.074 μg内线性关系良好(r=0.999 8)，平均加样回收率为98.17%；新橙皮苷在0.162~2.020 μg内线性关系良好(r=0.999 5)，平均加样回收率为100.61%。结论 本方法简便、准确，重复性好，可用于枳壳及其制剂血府逐瘀胶囊中柚皮苷和新橙皮苷的含量测定。

Abstract:

OBJECTIVE To establish an HPLC method for the determination of naringin and neohesperidin in Aurantii Fructus and its preparation Xuefu Zhuyu Capsules. METHODS The column was SunFire^TM C₁₈(250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water(20∶80) adjusted to pH 2.5 with phosphoric acid. The flow rate was 1.0 mL·min^-1 and the detection wavelength was at 283 nm. RESULTS The calibration curves were linear within the range of 0.166-2.074 μg(r=0.999 8) for naringin, 0.162-2.020 μg(r=0.999 5) for neohesperidin, respectively. The average recovery of them were 98.17% and 100.61%, respectively. CONCLUSION The method is simple, practicable, accurate and rapid. It can be applied to content determination of naringin and neohesperidin in Aurantii Fructus and Xuefu Zhuyu Capsules.