辛伐他汀联合辅酶Q10对慢性心衰大鼠心肌转化生长因子β1表达的影响

Effect of Simvastatin and Coenzyme Q10 on Expression of TGF-β1 in Rats with Chronic Heart Failure

关键词:  辛伐他汀  辅酶Q₁₀  慢性心力衰竭  转化生长因子β₁

Key words:  simvastatin  coenzyme Q₁₀  chronic heart failure  TGF-β₁

摘要:

目的 探讨辛伐他汀联合辅酶Q₁₀对慢性心力衰竭大鼠心肌转化生长因子β₁(transforming growth factor-β₁，TGF-β₁)表达的影响。方法 利用腹主动脉缩窄法制作SD大鼠慢性心力衰竭模型，随机分为模型组、辛伐他汀组、辅酶Q₁₀组、辛伐他汀+辅酶Q₁₀组，另设假手术组，每组8只大鼠，观察各组大鼠血流动力学参数(左室舒张末压、±dp/dt_max) 和左心室心肌组织病理学改变，SP免疫组化检测各组大鼠左心室心肌中TGF-β₁表达情况。结果 与模型组相比，辛伐他汀组、辅酶Q₁₀组和辛伐他汀+辅酶Q₁₀组左室舒张末压显著降低(P<0.01)，±dp/dt_max显著升高(P<0.01)，左心室肌TGF-β₁阳性表达水平显著降低(P<0.01)；联合用药较辛伐他汀组、辅酶Q₁₀改变更为显著(P<0.05)。结论 辛伐他汀与辅酶Q₁₀联合应用较两药单独应用改善心衰大鼠心功能、抑制心室重塑和减少TGF-β₁表达作用明显。

Abstract:

OBJECTIVE To study the effect of simvastatin and coenzyme Q10 on expression of transforming growth factor-β₁(TGF-β₁) in rats with chronic heart failure(CHF). METHODS SD rats were randomly divided into 5 groups: model group, simvastatin group, coenzyme Q₁₀, simvastatin combined coenzyme Q₁₀ group and sham-operated group. The CHF model was established with coarctation of abdominal aorta(CCA). Hemodynamic of left ventricular end-diastolic diameter press(LVEDP), left ventricular pressure rise and fall (±dp/dt_max) was tested; HE and Masson’s trichrome staining was carried out for morphology. Expression of TGF-β₁ in left ventricle was assayed by SP immunohistochemistry. RESULTS Compared with model group, LVEDP in other groups was significantly lower(P<0.01), ±dp/dt_max significantly higher(P<0.01); in addition, protein expression of TGF-β₁ in left ventricle tissue was also reduced, while simvastatin combined coenzyme Q₁₀ had more obvious effects on improving heart fouction of rats with cardiac remodeling and decreasing expression of TGF-β₁ protein. CONCLUSION Simvastatin combined with coenzyme Q₁₀ simvastatin can enhance the efficacy of single treatment on heart fouction, and protein expression of TGF-β₁.