白藜芦醇抑制高糖诱导的肾脏系膜细胞增殖的机制研究

Mechanism Study of Resveratrol Inhibited High Glucose-induced the Proliferation of Glomerular Mesangial Cell

关键词:  白藜芦醇  HBZY-1 cells(MCS)  增殖  Wnt/β-catenin

Key words:  resveratrol  HBZY-1 cells(MCS)  proliferation  Wnt/β-catenin

摘要:

目的 探讨白藜芦醇(resveratrol，RSV)对高糖诱导的大鼠肾脏系膜细胞HBZY-1 cells(MCS)增殖的影响，并探讨其潜在作用机制。方法 将对数生长期细胞随机分为对照组、高糖组、RSV组。各组细胞培养48 h后，利用MTT检测各组HBZY-1细胞增殖；RT-PCR检测Wnt、β-catenin、Collagen-Ⅳ、TNF-α、IL-6 和MCP-1基因表达；ELISA检测TNF-α、IL-6 和MCP-1表达；Western blotting检测Wnt、β-catenin和Collagen-Ⅳ蛋白的变化。结果 与对照组相比，高糖组细胞增殖率、Wnt、β-catenin、Collagen-Ⅳ、TNF-α、IL-6 和MCP-1表达水平均明显增高(P<0.05)。与高糖组相比，RSV组细胞增殖率、Wnt、β-catenin、p-p65、Collagen-Ⅳ、TNF-α、IL-6和MCP-1表达水平均明显降低(P<0.05)。结论 RSV可通过抑制炎症而减轻高糖诱导的肾脏系膜细胞增殖，其机制与抑制Wnt/β-catenin/p65信号通路激活有关。

Abstract:

OBJECTIVE To explore the effect and potentail mechanism of resveratrol(RSV) on the high glucose-induced the proliferation of glomerular mesangial cell HBZY-1(MCs) cultured under high glucose conditions. METHODS The HBZY-1(MCs) cells at logarithmic growth phase were randomly divided into control group, high glucose group and RSV group. The proliferation of the MCS cell was measured by MTT. The mRNA level of Wnt, β-catenin, Collagen-Ⅳ, TNF-α, IL-6 and MCP-1 were measured by RT-PCR. The expression of TNF-α, IL-6 and MCP-1 were measured by ELISA. The expression of Wnt, β-catenin and Collagen-Ⅳ were measured by Western blotting. RESULTS Compared with the control group, the proliferation rate of HBZY-1 cells , the expression of and protein level of Wnt, β-catenin, Collagen-Ⅳ, TNF-α, IL-6 and MCP-1 were significantly increased(P<0.05). Compared with the high glucose group, the proliferation rate of HBZY-1 cells, the expression of and protein level of Wnt, β-catenin, p-p65, Collagen-Ⅳ, TNF-α, IL-6 and MCP-1 were significantly decreased(P<0.05). CONCLUSION RSV can inhibit the proliferation of HBZY-1 cell by suppressing inflammation and the mechanism may be achieved through attenuating Wnt/β-catenin signal transduction pathway activation.