黑加仑籽与泽泻汤、山楂组方配伍降压调脂作用的拆方实验研究

Separated Prescription Research on Black Currant Seed and Zexie Decoction, Crataegi Fructus Combination about Depressurization and Adjusting Blood Lipid

关键词:  黑加仑籽  泽泻汤  山楂  析因分析  高血压复合高脂血症

Key words:  black currant seed  Zexie decoction  Crataegi Fructus  factorial design  compounding hypertension and hyperlipoidemia

摘要:

目的 观察黑加仑籽与泽泻汤、山楂组方配伍对肾性高血压复合高脂血症大鼠的收缩压及血脂的影响。方法 通过两肾一夹手术及高脂饲料喂养，建立肾性高血压复合高脂血症大鼠模型。选取造模成功的大鼠，采用析因设计进行拆方研究，按23析因法的设计，将大鼠分为8个组，同时设立阳性对照组和假手术组，分别给予不同的方剂组合，每天灌胃给药1次，共8周，除假手术组外，其余均高脂饲料喂养。观察药物对实验动物收缩压、血清总胆固醇(TC)、甘油三脂(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平的影响，并对肝、肾及胸主动脉组织切片做病理学观察。结果 拆方实验发现，本组方中起降压作用的组分为泽泻汤，黑加仑籽、泽泻汤和山楂均能显著性降低TC、TG、LDL-C，升高HDL-C。全方组优于其他各拆方组，并能减轻其脂肪肝，肾小球动脉硬化症状，胸主动脉中膜增厚症状。结论 本组方有很好的降压调脂作用，并对肝、肾及胸主动脉有较好的保护作用。

Abstract:

OBJECTIVE To observe the effect of black currant seed and Zexie decoction, Crataegi Fructus Combination on compounding hypertension and hyperlipoidemia rats in the influence ofsystolic blood pressure and blood lipid. METHODS Renal hypertensionwere fed high fat forage to set up the model of the rats of compounding renal hypertension and hyperlipoidemia. Using factorial design party and to determine factors and level number. According to factorial design 23, rats were randomly divided into 8 groups, and positive control group and sham operation group were set up. Each group was treated differently. The drugs were given by intragastric administration, at once per day for 8 weeks. SBP, TC, TG, LDL-C, HDL-C was measured to observe the effects of different medicine on the test animals. And pathology observation to the tissue slice of liver, kidney and thoracic aorta was done. RESULTS Separated prescription experiment found that only Zexie decoction had effect of depressurization(P<0.01). Black currant seed and Zexie Decoction, Hawthorn all could significantly reduce TC, TG, LDL-C(P<0.05), significantly increase HDL-C. The most effective group was not been split, which had a good depressurization and lipid-lowering effect, and could ease fatty liver and renal glomerulus arteriosclerosis symptom of compounding hypertension and hyperlipidemia rats, make dissepiment of glomerular arteriole and thickness of aortic media thinningz, and effectually protect liver, kidney and thoracic aorta so on capital organ. CONCLUSION The combinationhas effect of depressurization and adjusting blood lipid, and has better protection for liver, kidney and thoracic aorta.