补阳还五汤联合依达拉奉对小鼠急性脑缺血再灌注损伤的神经保护作用

Neural Protective Effects of Buyang Huanwu Decoction Combined with Edaravone on Mice of Acute Cerebral Ischemia-reperfusion Injury

关键词:  补阳还五汤  依达拉奉  脑缺血再灌注  细胞凋亡

Key words:  Buyang Huanwu decoction  edaravone  cerebral ischemia reperfusion  cellular apoptosis

摘要:

目的 研究补阳还五汤联合依达拉奉对小鼠脑缺血再灌注损伤模型的保护作用。方法 改良线栓法建立小鼠大脑中动脉缺血再灌注模型。小鼠随机分为假手术组、模型组、补阳还五汤组、依达拉奉组和补阳还五汤+依达拉奉组。术后1，3，7 d观察治疗后各组小鼠神经功能缺损评分。术后7 d，比较各组脑梗死体积大小，TUNEL法检测细胞凋亡。结果 与假手术组比较，模型组小鼠神经缺损评分、脑梗死体积、凋亡细胞数均显著增加(P<0.01)。与模型组比较，各用药组神经缺损评分、脑梗死体积、凋亡细胞数均显著减少(P<0.05)，且联合用药组以上指标均显著好于单用药组。结论 补阳还五汤与依达拉奉联合用药对小鼠脑缺血再灌注损伤有协同神经保护作用，比单药使用更有效地抑制细胞凋亡。

Abstract:

OBJECTIVE To explore the protective effects of Buyang Huanwu decoction(BYHWD) combined with edaravone(ED) on cerebral ischemia-reperfusion injury in mice. METHODS The middle cerebral artery occlusion/reperfusion model was established by the improved intraluminal filament technique in mice. The mice were randomly divided into sham operation group, model group, BYHWD group, ED group, BYHWD+ED group. The differences of neurologic impairment score were observe on 1st, 3rd and 7th day. The cerebral infarction volume was observed and the cellular apoptosis were compared by TUNEL method on the 7th day. RESULTS Compared with sham operation group, neurological deficit score, cerebral infarction volume and the cellular apoptosis increased significantly in model group(P<0.01). Compared with model group, the neurologic impairment score, cerebral infarction volume and the cellular apoptosis decreased significantly in each treated group(P<0.05). The above indexes of drug combination group were better than those of the single drug grup. CONCLUSION The combination of BYHWD and ED has a protective effect on cerebral ischemia-reperfusion injury, which is superior to the simple drug in inhibiting the cellular apoptosis.